FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, specifically to the production of immune cytokines; it can be used in medicine for the therapy of immune disorders. Proposed protein based on an antibody with grafted cytokine contains a variable region of a heavy chain (VH) containing complementarity determining regions (hereinafter – CDR), HCDR1 with SEQ ID NO: 17, HCDR2 with SEQ ID NO: 18, HCDR3 with SEQ ID NO: 19; and a variable region of a light chain (VL) containing LCDR1 with SEQ ID NO: 30, LCDR2 with SEQ ID NO: 31, LCDR3 with SEQ ID NO: 32; and an interleukin 2 (hereinafter – IL2) molecule grafted on HCDR1.
EFFECT: invention provides preferable binding and stimulation of signal transmission by means of a high-affinity receptor of IL2 relatively to a low-affinity receptor of IL2; increase or maintenance of Treg activity without increase in activity of CD8+ T-cells, Tcon, or NK-cells; improved half-life, stability, and production possibility compared to the known compositions based on recombinant human IL2.
26 cl, 20 dwg, 2 tbl, 19 ex
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