METHOD FOR OBTAINING A MEDICINAL SUBSTANCE FROM AMUR VELVET LEAVES Russian patent published in 2022 - IPC A61K36/756 B01D11/02 A61K127/00 

FIELD: chemical and pharmaceutical industry.

SUBSTANCE: invention relates to the chemical and pharmaceutical industry, in particular to a method for producing a medicinal substance obtained from Amur velvet leaves (Phellodendron amurence Rupr) and Amur Laval velvet leaves (Phellodendron amurence var. Lavallei (Dode) Spague) of the Rutaceae family and used in as an antiviral and antihepatotoxic drug Flacoside. The effect is achieved using a method for obtaining a medicinal substance from Amur velvet leaves (Phellodendron amurence Rupr) and Amur Laval velvet leaves (Phellodendron amurence var. Lavallei (Dode) Spague) of the Rutaceae family, including three-fold extraction of dried and crushed raw materials with aqueous ethyl alcohol at a ratio of raw material: extractant equal to 1:10, at a concentration of 40-60% at a temperature of 20-60°C for two hours, supply of fresh extractant for each subsequent extraction in an amount equal to the drained extraction, evaporation of the obtained extracts sequentially as they arrive up to a volume of 1/20, combining aqueous distillation concentrates and processing them four times with a mixture of 1,2-dichloroethane with ethyl alcohol in a ratio of 1:1:1, evaporation of organic solvent residues, alkalization of aqueous distillation residues with a 10-12% aqueous solution of sodium hydroxide up to pH 7-7.68, four-fold treatment of aqueous alkaline solutions of ethyl cetate in a ratio of 1:0.5, combining ethyl acetate extracts, washing with water, drying over sodium sulfate and evaporation, obtaining the technical product flacoside by crystallization from a mixture of ethyl acetate: water by cooling, followed by filtration and washing with chilled ethyl acetate, drying the technical product, recrystallization from aqueous ethyl alcohol and obtaining the finished flacoside.

EFFECT: development of an improved method for obtaining a flacoside substance from Amur velvet leaves, which has antiviral activity against herpes simplex virus and hepatitis C, due to the high content of the main active ingredient fellamurin of at least 96% (HPLC method).

1 cl, 1 tbl