METHOD FOR PRODUCING AN ANTI-INFLAMMATORY AGENT BASED ON MAMMALIAN SPLEEN PEPTIDES, AGENT PRODUCED THEREBY AND APPLICATION THEREOF Russian patent published in 2023 - IPC A61K35/26 C07K14/47 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to the field of biotechnology and discloses a method for producing an anti-inflammatory agent based on mammalian spleen peptides, involving the sequence of the following stages: mixing the minced mammalian spleen with an 0.1% chloride solution at the ratio of 2 pts. wt. of the solution to 1 pt. wt. of the minced spleen; fixing the large molecular weight fraction by acidifying the resulting homogenate while intensely stirring to a pH of 3.0±0.2 using a 5 M hydrochloric acid solution; extracting peptides by bringing the pH of the homogenate to a value of 8.0±0.2 using a 5 M sodium hydroxide solution; producing a clarified extract by freezing/exudation or flow centrifugation; precipitating the large molecular weight ballast by adding a 5 M sulphuric acid solution to the extract until a pH value of 2.5±0.2, ensuring ballast cutoff using a nutsch filter and obtaining an acidic filtrate; precipitating the sulphate by neutralising the filtrate with a 30% aqueous calcium hydroxide suspension to a pH value of 7.4±0.2, ensuring ballast cutoff using a nutsch filter and obtaining a neutral filtrate; precipitating the residual ballast and partially desalinating by adding a calculated volume of 96% ethanol up to 40% wt. to the filtrate, ensuring cutoff of the residual protein-saline precipitate using a nutsch filter and obtaining a composition of a cutaneous aqueous-alcohol lotion with systemic anti-inflammatory activity, containing a fraction of purified mammalian spleen peptides with a molecular weight not above 12 kDa at a concentration of 1e5% wt. to the total weight of the composition, 96% ethanol in the amount of 40% wt., sodium chloride in an amount of 0 to 0.5% wt., zinc chloride in an amount of 0 to 0.05% wt., calcium sulphate in an amount of 0 to 0.2% wt., water the rest, at a pH of 7.4±0.2. The group of inventions also relates to an anti-inflammatory agent based on mammalian spleen peptides in the form of a composition of the cutaneous aqueous-alcohol lotion with systemic anti-inflammatory activity.

EFFECT: anti-inflammatory therapeutic effect against the background of permanent forced superexpression of the strongest pro-inflammatory cytokine; more effective stimulation of immature (naïve) monocytes for Treg production; systemic anti-inflammatory therapeutic effect of the agent when used in the form of an aqueous-alcohol lotion applied to the skin; prevented development of psoriatic and rheumatoid arthritis with an efficacy of approx. 70%; possibility of achieving a tolerogenic anti-inflammatory therapeutic effect in a form that is suitable for outpatient individual administration; possibility of achieving a systemic tolerogenic anti-inflammatory therapeutic effect without undesirable side effects manifesting.

6 cl, 2 tbl, 10 dwg, 8 ex