FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. An H(+)/CL(-) exchange transporter consisting of the amino acid sequence represented by SEQ ID NO: 1 is proposed, in which cysteine, an amino acid corresponding to position 133 in SEQ ID NO: 3, is substituted for phenylalanine. A polynucleotide encoding the H(+)/Cl(-)exchange transporter, an Escherichia coli microorganism, which produces L-tryptophan and contains the H(+)/Cl(-) exchange transporter or a polynucleotide encoding the H(+)/Cl(-)exchange transporter, and a method for producing L-tryptophan are also proposed.
EFFECT: invention provides for increasing the L-tryptophan yield.
5 cl, 1 tbl, 2 ex
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Authors
Dates
2023-02-27—Published
2021-04-19—Filed