FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. A soluble pyridine nucleotide transhydrogenase comprising the amino acid sequence represented by SEQ ID NO: 1 is proposed, in which the alanine, an amino acid corresponding to position 145 in SEQ ID NO: 3, is replaced by valine. A polynucleotide encoding the soluble pyridinnucleotide transhydrogenase, an Escherichia coli microorganism, which produces L-tryptophan and contains the soluble pyridinnucleotide transhydrogenase or a polynucleotide encoding the soluble pyridinnucleotide transhydrogenase, and a method for producing L-tryptophan are also proposed.
EFFECT: invention provides for increasing the L-tryptophan yield.
5 cl, 1 tbl, 2 ex
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