FIELD: biotechnology.
SUBSTANCE: it can be used to increase the muscle mass of farm animals and poultry. An immunologically active recombinant GBD-ActRIIB protein is described containing a fragment of the ActRIIB receptor (type II activin receptor B) and a glucan-binding domain (GBD). A method has been developed for obtaining the recombinant GBD-ActRIIB protein in Escherichia coli BL21 cell extracts, which includes protein binding to an alpha-glucan-containing sorbent due to affinity interaction, subsequent washing of unbound bacterial proteins and isolation of the target product. An injectable preparation based on the recombinant GBD-ActRIIB protein suspended in an alpha-glucan-containing sorbent medium in an injectable liquid adjuvant was obtained. A method has been developed for using this drug, which includes subcutaneous or intramuscular injections twice with an interval of 20 days at a dose of 5-100 mcg of the recombinant GBD-ActRIIB protein per kilogram of animal or bird body weight.
EFFECT: technical result consists in the creation of a recombinant GBD-ActRIIB protein that is easy to purify and has sufficient immunogenicity against ActRIIB, as well as the creation of an injectable preparation containing an immunologically active recombinant GBD-ActRIIB protein, and its method of application.
6 cl, 4 tbl, 4 ex
Title |
Year |
Author |
Number |
RECOMBINANT GROWTH DIFFERENTIATION FACTOR 11 (GDF11), A METHOD FOR ITS PRODUCTION, AN INJECTABLE DRUG FOR INCREASING THE MUSCLE MASS OF MAMMALS AND POULTRY AS WELL AS A METHOD OF USING THE DRUG |
2020 |
- Lunin Vladimir Glebovich
- Kuznetsov Dmitrij Pavlovich
|
RU2750267C1 |
RECOMBINANT PROTEIN GBD-SSTAD-SSTAD, A METHOD FOR PRODUCTION AND USE THEREOF |
2019 |
- Lunin Vladimir Glebovich
- Yudin Sergej Mikhajlovich
- Reshetnik Vyacheslav Viktorovich
- Magataev Vali-Magomed Kadievich
|
RU2722849C1 |
COMBINED USE OF GDF TRAPS AND ERYTHROPOIETIN RECEPTOR ACTIVATORS TO INCREASE ERYTHROCYTE COUNT |
2020 |
- Seehra, Jasbir
- Pearsall, Robert, Scott
- Kumar, Ravindra
|
RU2814047C2 |
RECOMBINANT FUSED PROTEIN |
2019 |
- Maksimyak Sergej Petrovich
- Al-Shekhadat Ruslan Ismailovich
|
RU2732795C1 |
HERPES VACCINE |
2019 |
- Al-Shekhadat Ruslan Ismailovich
- Simbirtsev Andrei Semenovich
|
RU2731073C1 |
COMPOSITIONS AND METHODS FOR INTERNALIZATION OF ENZYMES |
2018 |
- Bejk, Endryu
- Signar, Katrin
- Shonkherr, Kristofer
- Kiratsus, Kristos
- Vang, Cheng
|
RU2806021C2 |
GENE THERAPY OF HYPOPHOSPHATEMIC DISEASES ASSOCIATED WITH FIBROBLAST GROWTH FACTOR 23 |
2020 |
- Rontsitti, Dzhuzeppe
- Zhoz, Luiza
- Sharl, Severin
- Mingotstsi, Frederiko
|
RU2815545C2 |
RECOMBINANT PLASMID DNA ENCODING CHIMERIC INTERFERON ALPHA2b, RECOMBINANT STRAIN OF YEAST P. PASTORIS X33 - PRODUCER OF CHIMERIC INTERFERON ALPHA2b, AND METHOD FOR PRODUCING THIS PROTEIN |
2020 |
- Beklemishev Anatolij Borisovich
- Pykhtina Mariya Borisovna
|
RU2764787C1 |
GENETIC CONSTRUCT CODING HUMAN YB-1 PROTEIN PRECURSOR, ESCHERICHIA COLI STRAIN - PRODUCER OF HUMAN YB-1 PROTEIN PRECURSOR, METHOD FOR MICROBIOLOGICAL SYNTHESIS OF SAID PRECURSOR |
2019 |
- Kozlov Dmitrij Georgievich
- Sannikova Evgeniya Pavlovna
- Bulushova Natalya Vladimirovna
- Zalunin Igor Arsenevich
|
RU2728237C1 |
ANTIBODIES TO MYOSTATIN AND THEIR APPLICATION METHODS |
2017 |
- Rujke Esinao
- Kuramoti Tajti
- Muramatsu Khiroyasu
- Ueyama Atsunori
|
RU2778945C2 |