GBO-ACTRIIB RECOMBINANT PROTEIN FOR INCREASING MUSCLE MASS IN FARM ANIMALS AND POULTRY Russian patent published in 2023 - IPC C07K19/00 C07K14/475 A61K38/18 A61P21/00 C12P1/00 

FIELD: biotechnology.

SUBSTANCE: it can be used to increase the muscle mass of farm animals and poultry. An immunologically active recombinant GBD-ActRIIB protein is described containing a fragment of the ActRIIB receptor (type II activin receptor B) and a glucan-binding domain (GBD). A method has been developed for obtaining the recombinant GBD-ActRIIB protein in Escherichia coli BL21 cell extracts, which includes protein binding to an alpha-glucan-containing sorbent due to affinity interaction, subsequent washing of unbound bacterial proteins and isolation of the target product. An injectable preparation based on the recombinant GBD-ActRIIB protein suspended in an alpha-glucan-containing sorbent medium in an injectable liquid adjuvant was obtained. A method has been developed for using this drug, which includes subcutaneous or intramuscular injections twice with an interval of 20 days at a dose of 5-100 mcg of the recombinant GBD-ActRIIB protein per kilogram of animal or bird body weight.

EFFECT: technical result consists in the creation of a recombinant GBD-ActRIIB protein that is easy to purify and has sufficient immunogenicity against ActRIIB, as well as the creation of an injectable preparation containing an immunologically active recombinant GBD-ActRIIB protein, and its method of application.

6 cl, 4 tbl, 4 ex