FIELD: biotechnology.
SUBSTANCE: essence of the invention consists in combining the alcohol precipitation method with ethanol at low temperatures with a three-stage chromatographic purification using the ProteinA sorbent in the first stage, a sorbent containing positively charged S-ligands (sulfopril), with a base matrix of a composite of polysaccharides (agarose) in the second stage, and a sorbent from agarose with hydrophobic ligands at the third stage, which makes it possible to obtain a homologous immunobiological preparation with a purity of the monomer fraction of at least 98%. The technical result of the invention is that the observance of these technological stages ensures the isolation of highly purified homologous immunoglobulin against COVID-19 from the blood plasma of convalescents with the content of the IgG monomer fraction in the final product of at least 98% and the titer of antibodies to the SARS-CoV-2 virus in the neutralization reaction not less than 1:512.
EFFECT: invention can be used for passive antibody therapy against the SARS-CoV-2 virus in the early stages of the disease.
1 cl, 1 tbl, 1 ex
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