Show metadata Hide metadata

(19)

(11)

2 822 761

(13)

(51)

IPC

C07K1/00(2006-01-01)

C07K1/22(2006-01-01)

C12Q1/6876(2018-01-01)

(21) (22)

Application

2021131096, 2021-10-25

(24)

Start date

2021-10-25

(22)

Actual filing date

2021-10-25

(45)

Published

2024-07-12

(72)

Inventor

Shuvalov Aleksej VitalevichShuvalova Ekaterina YurevnaAlkalaeva Elena Zinovevna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Nauki Institut Molekulyarnoj Biologii Im. V.A. Engelgardta Rossijskoj Akademii Nauk Ran)

METHOD OF PRODUCING AND PURIFYING FUNCTIONALLY ACTIVE RECOMBINANT NSP1 SARS-CoV-2 PROTEIN AND METHOD OF IDENTIFYING POTENTIAL ANTI-COVID-19 DRUG COMPOUNDS AIMED AT INHIBITING NSP1 Russian patent published in 2024 - IPC C07K1/00 C07K1/22 C12Q1/6876

Abstract RU 2822761 C2

FIELD: biotechnology.

SUBSTANCE: described is a group of inventions comprising a method of producing a highly purified, functionally active protein NSP1 SARS-CoV-2 and a method for testing the activity of the highly purified protein NSP1 SARS-CoV-2, obtained using the method described above, for inhibiting translation in an RRL translation system in vitro. Method of producing NSP1 SARS-CoV-2 protein involves creating an expression construct pET-6xHIS-SUMO-NSP1 using a set of primers with sequences SEQ ID NO: 1–4 and recovery of recombinant protein NSP1 SARS-CoV-2 during two rounds of metal-chelate affinity chromatography.

EFFECT: invention extends the range of methods for producing purified functionally active protein NSP1 SARS-CoV-2.

2 cl, 6 dwg, 3 ex

Similar patents RU2822761C2

Title	Year	Author	Number
PEPTIDE IMMUNOGENS AND A VACCINE COMPOSITION AGAINST CORONAVIRUS INFECTION COVID-19 USING PEPTIDE IMMUNOGENS	2020	Ryzhikov Aleksandr Borisovich Ryzhikov Evgenij Aleksandrovich Bogryantseva Marina Polikarpovna Gavrilova Elena Vasilevna Danilenko Elena Dmitrievna Imatdinov Ilnaz Ramisovich Maksyutov Rinat Amirovich Nechaeva Elena Avgustovna Popova Anna Yurevna Pyankov Oleg Viktorovich Pyankova Olga Grigorevna Susloparov Ivan Mikhajlovich	RU2738081C1
VACCINE COMPOSITION AGAINST COVID-19	2020	Ryzhikov Aleksandr Borisovich Ryzhikov Evgenij Aleksandrovich Bogryantseva Marina Polikarpovna Gavrilova Elena Vasilevna Danilenko Elena Dmitrievna Imatdinov Ilnaz Ramisovich Maksyutov Rinat Amirovich Nechaeva Elena Avgustovna Popova Anna Yurevna Pyankov Oleg Viktorovich Pyankova Olga Grigorevna Susloparov Ivan Mikhajlovich	RU2743595C1
PEPTIDE IMMUNOGENS AND VACCINE COMPOSITION AGAINST COVID-19 WITH THE USE OF PEPTIDE IMMUNOGENS	2020	Ryzhikov Aleksandr Borisovich Ryzhikov Evgenij Aleksandrovich Bogryantseva Marina Polikarpovna Gavrilova Elena Vasilevna Danilenko Elena Dmitrievna Imatdinov Ilnaz Ramisovich Maksyutov Rinat Amirovich Nechaeva Elena Avgustovna Popova Anna Yurevna Pyankov Oleg Viktorovich Pyankova Olga Grigorevna Susloparov Ivan Mikhajlovich	RU2743593C1
PEPTIDE IMMUNOGENS USED AS COMPONENTS OF VACCINE COMPOSITION AGAINST COVID-19	2020	Ryzhikov Aleksandr Borisovich Ryzhikov Evgenij Aleksandrovich Bogryantseva Marina Polikarpovna Gavrilova Elena Vasilevna Danilenko Elena Dmitrievna Imatdinov Ilnaz Ramisovich Maksyutov Rinat Amirovich Nechaeva Elena Avgustovna Popova Anna Yurevna Pyankov Oleg Viktorovich Pyankova Olga Grigorevna Susloparov Ivan Mikhajlovich	RU2743594C1
METHOD OF OBTAINING RECOMBINANT ANTIGEN MBP_RBD_6HIS OF SARS-CoV-2 VIRUS WITH C-TERMINAL AFFINITY TAG 6XHIS-TAG, INTENDED FOR USE AS COMPONENT OF REAGENT KIT FOR COVID-19 SERODIAGNOSTICS	2023	Petrov Aleksandr Anatolevich Sajfulina Natalya Aleksandrovna Plekhanova Tamara Mikhajlovna Pyshnaya Nina Savelevna Sizikova Tatyana Evgenevna Kovalchuk Elena Anatolevna Tselikov Evgenij Mikhajlovich Karulina Natalya Vasilevna Lebedev Vitalij Nikolaevich Borisevich Sergej Vladimirovich	RU2813324C1
AGENT FOR INHIBITION OF REPLICATION OF SARS-COV-2 VIRUS MEDIATED BY RNA INTERFERENCE	2020	Khaitov Musa Rakhimovich Shilovskij Igor Petrovich Kofiadi Ilya Andreevich Sergeev Ilya Viktorovich Kozlov Ivan Borisovich Smirnov Valerij Valerevich Kozhikhova Kseniya Vadimovna Koloskova Olesya Olegovna Andreev Sergej Mikhajlovich Zhernov Yurij Vladimirovich Nikonova Aleksandra Aleksandrovna	RU2733361C1
RECOMBINANT DNA ENSURING PRODUCTION OF THE RECOMBINANT PROTEIN COV1 EXHIBITING IMMUNOGENIC PROPERTIES AGAINST SARS-COV-2 VIRUS	2021	Koroleva Irina Vladimirovna Suvorov Aleksandr Nikolaevich Leonteva Galina Fedorovna Kramskaya Tatyana Anatolevna Desheva Yuliya Andreevna Shvedova Tamara Nikolaevna	RU2776484C1
SET OF OLIGONUCLEOTIDES AND METHOD OF MULTIPLEX POLYMERASE CHAIN REACTION IN REAL TIME FOR DETECTION OF RNA SARS-COV-2	2021	Abdullaev Adkhamzhon Odilovich Makarik Tatyana Viktorovna Sudarikov Andrej Borisovich Fevraleva Irina Serafimovna Glinshchikova Olga Anatolevna	RU2752902C1
METHOD OF USING RECOMBINANT PROTEINS SARS-COV-2 AS PART OF A TEST SYSTEM FOR ELISA TEST WITH DETERMINING IGM, IGG, IGA CLASS ANTIBODY LEVELS IN BLOOD SERUM/PLASMA OF COVID-19 PATIENTS	2020	Kostin Nikita Nikolaevich Bobik Tatyana Vladimirovna Tsabaj Polina Nikolaevna Skryabin Georgij Andreevich Balmasova Irina Petrovna Simonova Mariya Aleksandrovna Mokrushina Yuliana Anatolevna Smirnov Ivan Vitalevich Aleshenko Natalya Leonidovna Nikitin Aleksej Eduardovich Bykov Andrej Yurevich Chekhonin Vladimir Pavlovich Gabibov Aleksandr Gabibovich	RU2730897C1
METHOD OF OBTAINING LARGE FRAGMENT OF Bst POLYMERASE (OPTIONS)	2022	Cherkashina Anna Sergeevna Mikheeva Olga Olegovna Soloveva Elena Dmitrievna Pika Mariia Igorevna Petrov Vadim Viktorovich Krasovitov Kirill Vladimirovich Frolova Alina Iurevna Shemetova Anastasiia Fedorovna Cherkashin Evgenii Aleksandrovich Akimkin Vasilii Gennadevich	RU2809366C1

RU 2 822 761 C2

Authors

Shuvalov Aleksej Vitalevich

Shuvalova Ekaterina Yurevna

Alkalaeva Elena Zinovevna

Dates

2024-07-12—Published

2021-10-25—Filed