FIELD: biotechnology.
SUBSTANCE: method of obtaining a recombinant antigen MBP_RBD_6HIS SARS-COV-2 virus with a C-terminal affinity tag 6xhis-tag, which provides for the transformation of Escherichia Coli JM109 strain, MBP_RBD_6HIS plasmid DNA containing the gene, which encodes the MBP_RBD_6HIS antigen MBP_RBD_6HIS of vaccine virus with C-terminal affinity tag 6xhis-tag.
EFFECT: production of highly purified RBA MBP_RBD_6His of the SARS-CoV-2 virus with a C-terminal affinity tag 6xHis-tag.
1 cl, 5 dwg, 2 tbl, 5 ex
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