METHOD FOR CHROMATOGRAPHIC EXTRACTION AND PURIFICATION OF IMMUNOGLOBULINS Russian patent published in 2019 - IPC B01D15/08 C07K16/06 B01D15/42 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Method for isolating and purifying immunoglobulins involves dissolving precipitate A in a sodium acetate buffer solution, viral solvent-detergent inactivation and chromatographic purification in a system of three serially connected columns, first of which is filled with particles of hydrophobic polymer sorbent, second – particles of anion-exchange polymer sorbent, third – particles of sulphocationite. First column is disconnected from the system and directed for regeneration, and the third one – for immunoglobulin G isolation. Second column is washed with water and eluted in two stages. At the first stage, admixtures of ballast protein and polymer compounds are eluted, at the second stage, immunoglobulins A and M are eluted with a sodium-acetate buffer solution containing sodium chloride. Fraction containing immunoglobulins A and M is subjected to ultrafiltration and additional purification on column with anion-exchange sorbent, performing stepwise elution.

EFFECT: invention enables to obtain highly purified immunoglobulins with high output in a simple technological scheme.

5 cl, 6 ex