METHOD FOR CHROMATOGRAPHIC EXTRACTION OF IMMUNOGLOBULIN Russian patent published in 2012 - IPC B01D15/08 C07K16/06 

FIELD: chemistry.

SUBSTANCE: invention relates to liquid chromatography. Disclosed is a method for chromatographic extraction of immunoglobulin, involving dissolution of a blood plasma protein fraction in a buffer solution, said blood plasma protein fraction being a residue A of blood plasma alcohol fractionation via a Cohn process. The obtained solution undergoes preliminary purification in two series-connected columns filled with a hydrophobic sorbent and anionite, respectively, and then passing a buffer solution through said two columns. After collecting the purified liquid fraction, which contains immunoglobulin, it is taken for solvent-detergent viral inactivation and then for chromatographic purification, which is carried out in a system of three series-connected columns filled with anionite, hydrophobic sorbent and cationite, respectively. The immunoglobulin from the column filled with cationite is eluted and columns with the anionite and hydrophobic sorbent are taken for regeneration.

EFFECT: invention enables to extract immunoglobulin from crude material - a residue A, obtained from blood plasma alcohol fractionation via a Cohn process, with high output and purity of the end product.

8 cl, 2 ex