METHOD OF DETERMINING THE CONVERSION OF THE MOLECULAR BIOLOGICAL SUBTYPE OF THE TOTALITY OF CIRCULATING TUMOR CELLS RELATIVE TO THE MOLECULAR BIOLOGICAL SUBTYPE OF THE PRIMARY TUMOR IN PATIENTS WITH BREAST CANCER Russian patent published in 2023 - IPC G01N33/48 

FIELD: oncology.

SUBSTANCE: invention can be used to determine the conversion of the molecular biological subtype (MBP) of a set of circulating tumor cells (CTC) relative to the MBP of the primary tumor in patients with breast cancer. The MBP of the primary tumor is determined according to the regulations of the clinical recommendations of RUSSCO and the MBP of the CSC combination, for this, in the venous blood fraction enriched with leukocytes after erythrocyte sedimentation, the expression in nucleated cells of the common leukocyte marker CD45, EpCam, as well as MBP markers ER, PR, is determined by flow cytometry. HER2 and Ki67, the obtained values are compared with those in the main tumor node, and if a discrepancy between them is detected, the MBP conversion of the CTC set relative to the MBP of the primary tumor is determined.

EFFECT: proposed method of determining the MBP conversion of the CTC population in patients with breast cancer makes it possible to predict the effectiveness of the chemotherapy regimen used.

1 cl, 3 dwg, 2 ex