FIELD: biotechnology.
SUBSTANCE: invention relates to a system comprising the following: (a) Cas12b protein; (b) a guide RNA, wherein the guide RNA guides the Cas12b protein to specifically bind to target nucleic acid molecules; and (c) a nucleic acid probe that is single stranded DNA. Also the following is disclosed: a device that includes: (1) amplification reaction module — bypass cleavage reactions, and (2) signal detection module.
EFFECT: detecting the presence of target nucleic acid molecules in a sample, as well as detecting SNPs (single nucleotide polymorphisms).
24 cl, 27 dwg, 3 tbl, 11 ex
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