METHOD OF DETERMINING AND DETECTING HIV PROTEASE INHIBITORS IN BIOLOGICAL FLUIDS IN COMBINATIONS Russian patent published in 2023 - IPC G01N1/28 G01N30/90 A61K38/55 

FIELD: medicine.

SUBSTANCE: invention relates to a method of the determination and detection of saquinavir and ritonavir in biological fluids. A method of determination and detection in urine, plasma or saliva of saquinavir and ritonavir in combinations with tenofovir, lopinavir, isoniazid, phenazepam, diazepam, phenobarbital, cinnarizine, picamilon by chromatography in a thin layer of a sorbent on Sorbfil plates of a solution of analytes and standard samples of substances-witnesses, while preparing the test solution by isolating the substances to be determined from 10 ml of urine, plasma, saliva, 30 ml of butyl acetate and 10 ml of sodium chloride solution 20% at pH 3.0, chromatography is carried out in a thin layer of the sorbent in the solvent system ethyl acetate — acetone — ethanol 95% — glacial acetic acid with a solvent ratio of 40:40:2:1 by volume and the detection of zones of substances on a chromatogram in UV light.

EFFECT: above invention makes it possible to determine saquinavir and ritonavir isolated from biological fluids with butyl acetate and 20% sodium chloride solution at pH 3 by chromatography and UV light detection.

1 cl, 1 ex