FIELD: genetic engineering and biotechnology.
SUBSTANCE: invention relates to a de novo genetic construct that provides heterologous expression of the S-protein receptor-binding domain in a fused polypeptide chain with a nucleocapsid protein.
EFFECT: invention can be used in the heterologous synthesis of the said chimeric protein in recombinant producer strains, as well as in obtaining its homogeneous preparation suitable for structural and biophysical studies.
1 cl, 4 dwg
| Title |
Year |
Author |
Number |
| ARTIFICIAL GENE CODING A BICISTRONIC STRUCTURE FORMED BY RECEPTOR-BINDING DOMAIN SEQUENCES OF THE GLYCOPROTEIN S OF THE SARS-COV-2 CORONAVIRUS, P2A-PEPTIDE AND GLYCOPROTEIN G VSV, RECOMBINANT PLASMID PSTEM-RVSV-STBL_RBD_SC2, PROVIDING EXPRESSION OF ARTIFICIAL GENE, AND A RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS RVSV-STBL_RBD_SC2, USED TO CREATE A VACCINE AGAINST SARS-COV-2 CORONAVIRUS |
2020 |
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- Tishin Anton Evgenevich
- Pyankov Oleg Viktorovich
- Gavrilova Elena Vasilevna
- Maksyutov Rinat Amirovich
|
RU2733831C1 |
| ARTIFICIAL GENE STBL_RBD_TRM_SC2, CODING A BICISTRONIC STRUCTURE FORMED BY THE SARS-COV-2 CORONAVIRUS GLYCOPROTEIN S RECEPTOR-BINDING DOMAIN SEQUENCES, TRANSMEMBRANE REGION, P2A-PEPTIDE AND GLYCOPROTEIN G VSV, RECOMBINANT PLASMID PSTEM-RVSV-STBL_RBD_TRM_SC2, PROVIDING EXPRESSION OF ARTIFICIAL GENE, AND A RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS RVSV-STBL_RBD_TRM_SC2, USED TO CREATE A VACCINE AGAINST SARS-COV-2 CORONAVIRUS |
2020 |
- Imatdinov Ilnaz Ramisovich
- Bochkareva Mariya Dmitrievna
- Prudnikova Elena Yurevna
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|
RU2733832C1 |
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2021 |
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|
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2023 |
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|
RU2816175C1 |
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2020 |
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|
RU2733834C1 |
| RECOMBINANT PLASMID pVEAL3-XBB.1.5, WHICH PROVIDES SYNTHESIS AND SECRETION OF RECEPTOR-BINDING DOMAIN RBD S OF SARS-CoV-2 PROTEIN OF VARIANT XBB.1.5, AND RECOMBINANT STRAIN CHO-XBB.1.5 - PRODUCER OF RECEPTOR-BINDING DOMAIN RBD S PROTEIN SARS-CoV-2 VERSION XBB.1.5 INTENDED FOR PRODUCING IMMUNOBIOLOGICAL PREPARATIONS |
2024 |
- Isaeva Anastasiya Aleksandrovna
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|
RU2839373C1 |
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2020 |
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|
RU2744274C1 |
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2023 |
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|
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2021 |
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|
RU2762962C1 |
| POLYPEPTIDE MODULE FOR BINDING CONSERVATIVE EPITOPE OF RECEPTOR-BINDING DOMAIN OF SPIKE PROTEIN OF SARS-CoV-2 CORONAVIRUS |
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