FIELD: biotechnologies.
SUBSTANCE: what is described is a recombinant plasmid genetic construct pVEAL3-XBB.1.5 providing synthesis and secretion of the receptor-binding domain RBD S protein of SARS-CoV-2 variant XBB.1.5 in CHO-K1 cells having the nucleotide sequence SEQ ID NO: 1, comprising elements in accordance with the physical and genetic map presented in Fig. 1. Recombinant strain of cells of Chinese hamster ovary CHO-XBB.1.5 – producer of receptor-binding domain RBD S protein of SARS-CoV-2 variant XBB.1.5 – is obtained by co-transfection of said cells with recombinant plasmid pVEAL3-XBB.1.5 according to cl. 1 and plasmid pCMV(CAT)T7-SB100, encoding transposase SB 100.
EFFECT: what is described is a recombinant plasmid genetic construct pVEAL3-XBB.1.5 providing synthesis and secretion of the receptor-binding domain RBD S protein of SARS-CoV-2 variant XBB.1.5 in CHO-K1 cells.
2 cl, 2 dwg, 1 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| INTEGRATIVE PLASMID VECTOR pVEAL3-RBDdel, PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT PROTEIN OF RECEPTOR-BINDING DOMAIN RBDdelta OF CORONAVIRUS SARS-CoV-2 IN MAMMALIAN CELLS, RECOMBINANT CELL LINE STRAIN CHO-K1-RBDdelta AND RECOMBINANT PROTEIN RBDdelta SARS-CoV-2 PRODUCED BY CELL LINE STRAIN | 2023 |
|
RU2816175C1 |
| INTEGRATIVE PLASMID VECTOR PVEAL2-S-RBD, PROVIDING THE EXPRESSION AND SECRETION OF THE RECOMBINANT RECEPTOR-BINDING DOMAIN (RBD) OF THE SARS-COV-2 CORONAVIRUS IN MAMMALIAN CELLS, THE RECOMBINANT CHO-K1-RBD CELL LINE STRAIN AND THE RECOMBINANT SARS-COV-2 RBD PROTEIN PRODUCED BY THE SPECIFIED STRAIN OF THE CELL LINE CHO-K1-RBD | 2021 |
|
RU2752858C1 |
| RECOMBINANT SARS-CoV-2 S PROTEIN RBD-BINDING PROTEIN | 2022 |
|
RU2778942C1 |
| HEAVY-CHAIN MONOCLONAL ANTIBODIES SPECIFICALLY BINDING TO S PROTEIN OF SARS-CoV-2 VIRUS, AND METHOD OF USING THEM FOR TREATMENT OF DISEASES CAUSED BY DIFFERENT VARIANTS OF SARS-CoV-2 VIRUS | 2024 |
|
RU2836313C1 |
| RECOMBINANT PLASMID PVBL-RBDOMIK PROVIDING SYNTHESIS AND SECRETION OF THE RECOMBINANT RECEPTOR-BINDING DOMAIN (RBD) OF THE SARS-COV-2 CORONAVIRUS LINE B.1.1.529 IN MAMMALIAN CELLS. | 2021 |
|
RU2772905C1 |
| RECOMBINANT PLASMID PVBL-RBDDELTA PROVIDING SYNTHESIS AND SECRETION OF THE RECOMBINANT RECEPTOR-BINDING DOMAIN (RBD) OF THE SARS-COV-2 CORONAVIRUS LINE B.1.617.2 IN MAMMALIAN CELLS | 2021 |
|
RU2772904C1 |
| Method for producing Chinese hamster ovary cell strain, being a producer of recombinant SARS-CoV-2 virus protein RBD, Chinese hamster ovary cell strain, producer of recombinant SARS-CoV-2 protein RBD, method for producing recombinant SARS-CoV-2 virus protein RBD, test system for enzyme immunoassay of human serum or plasma, and use thereof | 2020 |
|
RU2723008C1 |
| PLASMID GENETIC CONSTRUCT pET21a_SKP, WHICH PROVIDES EXPRESSION IN ESCHERICHIA COLI PROKARYOTIC SYSTEM OF RECOMBINANT SKP PROTEIN, AND RECOMBINANT PROTEIN SKP HAVING BROADLY NEUTRALIZING PROPERTIES OF SINGLE-DOMAIN NANOANTIBODY AGAINST SARS-CoV-2 | 2024 |
|
RU2839376C1 |
| RECOMBINANT RBD S-PROTEIN OF CORONAVIRUS SARS-CoV-2 AND METHOD FOR ITS PRODUCTION | 2023 |
|
RU2833839C1 |
| IMMUNOLIPOSOME WHICH BINDS TO RBD OF S-PROTEIN SARS-CoV-2 | 2023 |
|
RU2827165C1 |
