FIELD: genetic engineering.
SUBSTANCE: invention relates to genetic engineering, biotechnology and medicine. The integrative plasmid vector pVEAL2-S-RBD, which provides expression and secretion of the RBD protein of the SARS-CoV-2 coronavirus in mammalian cells, has a size of 9910 bp, the nucleotide sequence SEQ ID No.2 and contains elements in accordance with the physical and genetic map shown in Fig.1. Also created a recombinant strain of the Chinese hamster ovary cell line CHO-K1-RBD - the producer of the recombinant RBD protein of the SARS-CoV-2 virus containing the integrative plasmid vector pVEAL2-S-RBD according to claim 1, having the nucleotide sequence of SEQ ID NO: 2, providing the expression and secretion of the RBD protein of the SARS-CoV-2 coronavirus and deposited under number 322 in the collection of cell cultures of the FBSI SSC VB "Vector" Rospotrebnadzor (certificate of deposit is attached). Also obtained recombinant protein RBD coronavirus SARS-CoV-2, produced by the recombinant strain of Chinese hamster ovary cells CHO-K1-RBD according to claim 2 and having the amino acid sequence of SEQ ID NO: 3. The invention can be used to create vaccines.
EFFECT: invention increases the yield of the target recombinant protein RBD SARS-CoV-2.
3 cl, 5 dwg, 1 tbl, 5 ex
