DEVICES FOR AUTOMATIC CELL-FREE PRODUCTION OF PROTEINS AND METHODS FOR PROTEINS PRODUCTION USING THIS PRODUCT Russian patent published in 2017 - IPC C07K1/14 C12M1/38 

FIELD: biotechnology.

SUBSTANCE: automated system for cell-free protein production, a cell-free method for protein production, and a reaction set for automated cell-free protein production are proposed. The system includes a protein expression reaction unit, a reaction temperature control unit, an array of pipettes, a pipette array movement unit, protein purification unit, multiwell plates attachment unit with a multiwell plate. At that, the protein reaction expression unit comprises a reaction vessel with a dialysis tube. Protein purification unit comprises a device for magnetic field application to attach the target protein to magnetic microspheres with subsequent magnetic microspheres attachment to the wall, and the multiwell plate is provided with solutions required for proteins production. The method comprises the stages of mixture preparation for cell-free protein expression, protein expression, protein purification using magnetic particles and purified protein dialysis. The reaction set comprises a set of multiwell plates with individual wells for storage of the solution with amino acids necessary for transcription and translation, a power source and buffer, dialysis tubes with a dialysis membrane.

EFFECT: increased protein expression level.

24 cl, 11 dwg, 8 ex