BIOSENSOR BASED ON STAPHYLOCOCCUS AUREUS CELLS STABLY PRODUCING GREEN FLUORESCENT PROTEIN FOR PERFORMING ULTRAHIGH-PRODUCTIVITY SCREENING Russian patent published in 2020 - IPC C12N1/21 C12N15/63 C12R1/445 

Abstract RU 2722627 C2

FIELD: biotechnology.

SUBSTANCE: disclosed is a producer strain based on the strain Staphylococcus aureus NCTC 8325 transformed with the expression vector plasmid pALC1420, containing nucleotide sequences of constitutive promoter sar1, green fluorescent protein gene GFP, cat resistance to chloramphenicol gene.

EFFECT: invention provides stable intracellular production of green fluorescent protein GFP.

1 cl, 3 dwg, 2 ex

Similar patents RU2722627C2

Title Year Author Number
BIOSENSOR BASED ON ESCHERICHIA COLI CELLS PRODUCING FLUORESCENT PROTEIN KATUSHKA2S, FOR PERFORMING ULTRA-HIGH-PRODUCTIVITY SCREENING 2017
  • Terekhov Stanislav Sergeevich
  • Smirnov Ivan Vitalevich
  • Gabibov Aleksandr Gabibovich
RU2687046C1
REPORTER MONOCLONAL CELL LINE HEK-293/5-HT4R, EXPRESSING MURINE RECOMBINANT SEROTONIN RECEPTOR TYPE 5HT4 2022
  • Rogachevskaya Olga Anatolevna
  • Kotova Polina Dmitrievna
  • Cherkashin Aleksandr Pavlovich
  • Kabanova Nataliya Vladimirovna
RU2817234C2
CELL-FREE PROTEIN SYNTHESIS SYSTEM BASED ON STAPHYLOCOCCUS AUREUS CELLS, A METHOD OF PROTEIN SYNTHESIS BASED ON STAPHYLOCOCCUS AUREUS CELLS USING A CELL-FREE PROTEIN SYNTHESIS SYSTEM BASED ON STAPHYLOCOCCUS AUREUS CELLS AND A METHOD OF DETECTING PROTEIN SYNTHESIS INHIBITORS USING IT 2022
  • Golubev Aleksandr Aleksandrovich
  • Aleksandrova Natalia Mikhailovna
  • Validov Shamil Zavdatovich
  • Iusupov Marat Miratovich
  • Usachev Konstantin Sergeevich
RU2802080C1
NUCLEIC ACID ENCODING FRET-BASED FAR-RED BIOSENSOR FOR INTRACELLULAR CASPASE 3 ACTIVITY MEASUREMENT 2013
  • Luk'Janov Konstantin Anatol'Evich
  • Zlobovskaja Ol'Ga Anatol'Evna
RU2535981C1
ISOLATED NUCLEIC ACID CODING FLUORESCENT BIOSENSOR FOR DETECTION OF HYDROGEN PEROXIDE, EXPRESSION CASSETTE, CELL THAT PRODUCES BIOSENSOR, ISOLATED FLUORESCENT BIOSENSOR FOR DETECTION OF HYDROGEN PEROXIDE, ISOLATED NUCLEIC ACID THAT CODES FLUORESCENT BIOSENSOR, EFFICIENTLY FUSED WITH NUCLEIC ACID THAT CODES SIGNAL OF INTRACELLULAR LOCALISATION 2011
  • Bilan Dmitrij Sergeevich
  • Belousov Vsevolod Vadimovich
  • Luk'Janov Sergej Anatol'Evich
RU2493260C2
BACTERIAL STRAIN ESCHERICHIA coli/pTdcR-turboYFP, HAVING SENSITIVITY TO TERAHERTZ RADIATION 2018
  • Serdyukov Danil Sergeevich
  • Goryachkovskaya Tatyana Nikolaevna
  • Rozanov Aleksej Sergeevich
  • Meshcheryakova Irina Anatolevna
  • Peltek Sergej Evgenevich
RU2691308C1
CHIMERIC PROTEIN BEING FLUORESCENT BIOSENSOR FOR SIMULTANEOUS DETECTION OF HYDROGEN PEROXIDE AND PHOSPHATIDYL INOSITOL-3,4,5-TRIPHOSPHATE, NUCLEINIC ACID CODING SUCH PROTEIN, EXPRESSION CASSETTE AND EUCARIOTIC HOST CELL 2012
  • Belousov Vsevolod Vadimovich
  • Mishina Natalija Mikhajlovna
  • Chudakov Dmitrij Mikhajlovich
RU2498996C1
RED FLUORESCENT BIOSENSOR FOR DETECTION OF HYDROGEN PEROXIDE IN LIVING CELLS 2013
  • Belousov Vsevolod Vadimovich
  • Ermakova Julija Gennadievna
  • Enikolopov Grigorij Nikolaevich
RU2535336C1
BIOSENSOR FOR DETECTION OF HYDROGEN PEROXIDE IN LIVE CELLS, POSSESSING HIGHER STABILITY TO pH CHANGE 2009
  • Belousov Vsevolod Vadimovich
RU2434943C2
ISOLATED NUCLEIC ACID CODING FLUORESCENT BIOSENSOR, EXPRESSION CASSETTE, CELL, PRODUCING FLUORESCENT BIOSENSOR, ISOLATED FLUORESCENT BIOSENSOR 2012
  • Bilan Dmitrij Sergeevich
  • Belousov Vsevolod Vadimovich
  • Luk'Janov Sergej Anatol'Evich
RU2515903C2

RU 2 722 627 C2

Authors

Terekhov Stanislav Sergeevich

Smirnov Ivan Vitalevich

Gabibov Aleksandr Gabibovich

Dates

2020-06-02Published

2017-12-28Filed