FIELD: biotechnology.
SUBSTANCE: following is proposed: a set of oligodeoxyribonucleotide primers VT-BRT-F 5'-CGGAGGGCTTGTAGCTCAG-3', VT-BRT-R 5'-CTGGTGGGCCTGGGAG-3' and fluorescently labeled probe VT-BRT-P 5'-(R6G)-CCTCCGACCTCACGCTTATCAAGC-(BHQ2)-3' to the 16S ribosomal RNA gene fragment of Bartonella Henselae, Bartonella clarridgeiae, Bartonella koehlerae for the detection of pathogenic feline Bartonella.
EFFECT: set of oligodeoxyribonucleotide primers and a fluorescently labeled probe makes it possible to identify in real time the DNA fragment of the 16S ribosomal RNA gene of Bartonella Henselae, Bartonella clarridgeiae, Bartonella koehlerae, which has a higher homology to the currently circulating pathogenic feline Bartonella, thus increasing the reliability and reliability of the tests.
1 cl, 3 tbl, 3 dwg
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Year |
Author |
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