FIELD: veterinary.
SUBSTANCE: set of primers for detecting the genetic material of feline coronavirus/feline infectious peritonitis virus (FCoV, FIP) in clinical samples. The presented set of oligodeoxyribonucleotide primers and a fluorescently labeled DNA probe contains a pair of oligonucleotides with the activity of forward and reverse primers in the polymerase chain reaction, as well as one fluorescently labeled DNA probe. The present invention allows more authentic and reliable detection of the genetic material of the feline coronavirus/feline infectious peritonitis virus (FCoV, FIP).
EFFECT: invention makes it possible to detect a fragment of the conserved region of the N gene encoding the nucleocapsid protein of both the feline coronavirus (FCoV) and the feline infectious peritonitis virus (FIP), which is common for all variants of this pathogen, and allow to identify animals infected with any variant of the feline coronavirus (FCoV) virus and feline infectious peritonitis virus (FIP) at any stage of the disease, increases the authenticity and reliability of the tests.
1 cl, 3 tbl, 3 dwg
Authors
Dates
2023-09-05—Published
2022-10-26—Filed