METHOD OF GENOTYPING TLR2 GENE USING RS5743708 POLYMORPHISM AND A SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR ITS IMPLEMENTATION Russian patent published in 2023 - IPC C12Q1/6804 

FIELD: biotechnology.

SUBSTANCE: following has been proposed: a method of genotyping G and A alleles of TLR2 gene using rs5743708 polymorphism, including DNA extraction from biological material, real-time PCR using a reaction mixture containing a set of nucleotide primers and probes of the following SEQ ID NO: 1–4, amplification, analysis and interpretation of the results, where the presence of G allele of TLR2 gene for rs5743708 polymorphism is determined by assessing the accumulation curves of fluorescent signals through the channel for the FAM fluorophore, and the presence of A allele of TLR2 gene in the biological material sample for rs5743708 polymorphism is determined by assessing the curves accumulation of fluorescent signals through the channel for the R6G fluorophore. A set of oligonucleotide primers and probes for use in the genotyping method is proposed.

EFFECT: invention makes it possible to detect rs5743708-G and rs5743708-A alleles of TLR2 gene in samples of biological material using real-time PCR.

5 cl, 1 tbl, 6 ex