FIELD: biotechnology.
SUBSTANCE: following has been proposed: a method of genotyping A and G alleles of TLR6 gene using rs5743810 polymorphism, including DNA extraction from biological material, real-time PCR using a reaction mixture containing a set of nucleotide primers and probes of the following SEQ ID NO: 1–4, amplification, analysis and interpretation of the results, where the presence of A allele of TLR6 gene for rs5743810 polymorphism is determined by assessing the accumulation curves of fluorescent signals through the channel for the FAM fluorophore, and the presence in the environmental material sample of G allele of TLR6 gene for rs5743810 polymorphism is determined by assessing the curves accumulation of fluorescent signals through the channel for the R6G fluorophore. A set of oligonucleotide primers and probes for use in the genotyping method is proposed.
EFFECT: invention makes it possible to detect rs5743810-A and rs5743810-G alleles of TLR6 gene in samples of biological material using real-time PCR.
5 cl, 1 tbl, 6 ex
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