pGEM-TCRAC-sjTREC-sjKREC RECOMBINANT PLASMID DNA CONTAINING NUCLEOTIDE SEQUENCES OF THE T-LYMPHOCYTE Α-RECEPTOR GENE LOCUS AND SIGNAL FRAGMENTS OF THE T- AND B-CELL RECEPTOR EXCISION RINGS Russian patent published in 2023 - IPC C12Q1/6809 C12N15/70 

FIELD: genetic engineering; molecular and cellular biology.

SUBSTANCE: invention is intended to assess the specificity and sensitivity of primers and probes developed to estimate the number of copies of T-lymphocyteα-receptor and T-cell receptor excision ring signaling fragment in human DNA samples.

EFFECT: proposed pGEM-TCRAC-sjTREC-sjKREC plasmid DNA acts as a standardized positive control DNA sample when assessing the number of copies of the T-lymphocyte geneα-receptor and signal fragments of excision rings of T- and B-cell receptors when performing digital PCR with absolute quantification in biological samples of human DNA.

1 cl, 5 dwg, 1 ex