FIELD: genetic engineering; molecular and cellular biology.
SUBSTANCE: invention is intended to assess the specificity and sensitivity of primers and probes developed to estimate the number of copies of T-lymphocyteα-receptor and T-cell receptor excision ring signaling fragment in human DNA samples.
EFFECT: proposed pGEM-TCRAC-sjTREC-sjKREC plasmid DNA acts as a standardized positive control DNA sample when assessing the number of copies of the T-lymphocyte geneα-receptor and signal fragments of excision rings of T- and B-cell receptors when performing digital PCR with absolute quantification in biological samples of human DNA.
1 cl, 5 dwg, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR QUANTITATIVE ASSESSMENT OF T AND B-LYMPHOCYTES BY CONTENT OF EXCISION RINGS TREC AND KREC IN DNA SAMPLES USING DIGITAL POLYMERASE CHAIN REACTION | 2023 |
|
RU2822204C1 |
| METHOD OF DIAGNOSING CONDITION OF IMMUNE SYSTEM OF PATIENT AND SET OF PRIMERS, PROBES AND STANDARD SAMPLES FOR QUANTITATIVE ESTIMATION OF DNA MOLECULES TREC, KREC AND NUMBER OF GENOME EQUIVALENTS OF DNA | 2015 |
|
RU2587540C1 |
| METHOD FOR LABORATORY PERSONALIZED DIAGNOSTICS OF THE STATE OF IMMUNITY OF PATIENTS AND A SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS, AND FLUORESCENTLY LABELED PROBES, AND STANDARD SAMPLES | 2022 |
|
RU2786211C1 |
| METHOD FOR LABORATORY PERSONALIZED DIAGNOSTICS OF THE IMMUNE CONDITION OF NEWBORNS AND SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELED PROBES | 2019 |
|
RU2756979C2 |
| METHOD OF DNA EXTRACTION, SUITABLE FOR CONDUCTING QUANTITATIVE REAL-TIME PCR, FROM DRY BLOOD SPOTS OF NEWBORNS | 2017 |
|
RU2645089C1 |
| RECOMBINANT PLASMID DNA pSNCA-C-3XFLAG-2XST-DONOR PROVIDING STABLE DOXYCYCLINE-DRIVEN EXPRESSION OF ALPHA-SYNUCLEIN CHIMERIC PROTEIN IN HUMAN CELL CULTURES | 2022 |
|
RU2795156C1 |
| MONOMOLECULAR CHIMERIC T-CELL RECEPTOR TO CANCER ANTIGEN CA125 | 2018 |
|
RU2747095C2 |
| METHOD FOR MEASURING NUMBER OF COPIES OF HUMAN MITOCHONDRIAL DNA BY DIGITAL POLYMERASE CHAIN REACTION | 2021 |
|
RU2755663C1 |
| MONOMOLECULAR CHEMERIC T-CELLULAR RECEPTOR TO CANCER-EMBRYONIC ANTIGEN | 2015 |
|
RU2650858C2 |
| A SET FOR DETERMING THE ABUNDANCE OF THE INSERTIONOF THE CONSTRUCT OF INTEREST INTO THE AAVS1LOCUS OF THE HUMAN GENOME | 2021 |
|
RU2786396C1 |
