FIELD: molecular biology.
SUBSTANCE: described is a method for quantitative assessment of human T and B lymphocytes by digital polymerase chain reaction, characterized by the fact that an accurate assessment of the number of human T and B-lymphocytes in a biological sample is achieved by counting copies of signal fragments of excision rings TREC and KREC of genes, as well as a gene.
EFFECT: high accuracy of quantitative assessment of T and B-lymphocytes in human biological samples of interest.
1 cl, 3 dwg
Title | Year | Author | Number |
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pGEM-TCRAC-sjTREC-sjKREC RECOMBINANT PLASMID DNA CONTAINING NUCLEOTIDE SEQUENCES OF THE T-LYMPHOCYTE Α-RECEPTOR GENE LOCUS AND SIGNAL FRAGMENTS OF THE T- AND B-CELL RECEPTOR EXCISION RINGS | 2022 |
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Authors
Dates
2024-07-03—Published
2023-11-16—Filed