FIELD: biotechnology.
SUBSTANCE: method for genetic modification of a primary NK cell is disclosed, including: obtaining a guide RNA (gRNA) specific for a target DNA sequence, and introducing a ribonucleoprotein (RNP) complex containing a Class 2 CRISPR/Cas endonuclease bound into the complex into the target NK cell by electroporation with the corresponding CRISPR/Cas guide RNA hybridizing the target sequence within the genomic DNA of the NK cell. Also a method for the adaptive transfer of engineered NK cells to a subject in need thereof, comprising: obtaining a target NK cell to be modified, obtaining a gRNA specific for the target DNA sequence, introducing an RNP complex into the target NK cell by electroporation, and transferring the engineered NK cells into the subject's body.
EFFECT: effectively used in cancer immunotherapy.
13 cl, 16 dwg, 6 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHODS OF PRODUCING CELLS EXPRESSING RECOMBINANT RECEPTOR AND RELATED COMPOSITIONS | 2019 |
|
RU2835579C2 |
| ARTIFICIALLY CREATED SC FUNCTION CONTROL SYSTEM | 2017 |
|
RU2768043C2 |
| METHODS AND COMPOSITIONS FOR MODIFYING TARGET LOCUS | 2015 |
|
RU2704283C2 |
| METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATION USING PAIRED GUIDE RNAS | 2015 |
|
RU2734770C2 |
| TARGETING SRC-3 IN IMMUNE CELLS AS IMMUNOMODULATORY THERAPEUTIC AGENT FOR TREATING CANCER | 2021 |
|
RU2828447C1 |
| ARTIFICIAL GENOME MODIFICATION FOR GENE EXPRESSION REGULATION | 2018 |
|
RU2767201C2 |
| METHODS OF OVERCOMING IMMUNOLOGICAL TOLERANCE USING MULTIPLE TARGETING RNAS | 2017 |
|
RU2829155C2 |
| WAYS TO OVERCOME IMMUNOLOGICAL TOLERANCE USING MULTIPLE GUIDE RNAS | 2017 |
|
RU2745563C2 |
| COMPOSITIONS AND METHODS FOR TREATING HEMOGLOBINOPATHIES | 2018 |
|
RU2834231C2 |
| NUCLEASE-MEDIATED DNA ASSEMBLY | 2015 |
|
RU2707911C2 |
