FIELD: biotechnology.
SUBSTANCE: invention refers to biotechnology and molecular biology. Disclosed is an in vitro method of assembling nucleic acids using a nuclease agent - a complex of gRNA-Cas, nuclease with zinc fingers or effector nuclease.
EFFECT: invention can be used in applied biotechnology and medicine due to increased efficiency of producing long nucleic acids.
43 cl, 19 dwg, 1 tbl, 14 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHODS AND COMPOSITIONS FOR MODIFYING TARGET LOCUS | 2015 |
|
RU2704283C2 |
| METHODS FOR SEAMLESS INTRODUCTION OF TARGET MODIFICATIONS TO DIRECTIONAL VECTORS | 2020 |
|
RU2771374C1 |
| METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATION USING PAIRED GUIDE RNAS | 2015 |
|
RU2734770C2 |
| METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATIONS AND THEIR APPLICATION METHODS | 2015 |
|
RU2771532C2 |
| METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATION BY SINGLE-STEP MULTIPLE TARGETING | 2015 |
|
RU2707137C2 |
| METHODS AND COMPOSITIONS FOR PRODUCING OR MAINTAINING PLURIPOTENT CELLS | 2015 |
|
RU2706965C2 |
| METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATIONS AND METHODS FOR USE THEREOF | 2015 |
|
RU2711740C2 |
| CRISPR/CAS PLATFORM FOR EXCLUSIVE SCREENING FOR DETECTING GENETIC VULNERABILITIES ASSOCIATED WITH TAU PROTEIN AGGREGATION | 2020 |
|
RU2808829C2 |
| DNA-CUTTING AGENT BASED ON Cas9 PROTEIN FROM THE BACTERIUM STREPTOCOCCUS UBERIS NCTC3858 | 2022 |
|
RU2788197C1 |
| CRISPR/CAS SCREENING PLATFORM FOR IDENTIFICATION OF GENETIC MODIFIERS OF SEEDING OR AGGREGATION OF TAU PROTEIN | 2020 |
|
RU2819350C2 |
