FIELD: biotechnology; medicine; cosmetology.
SUBSTANCE: invention relates to the field of biotechnology, medicine and cosmetology. Deamidated collagen biomatrix in the form of a whole fibrous sheet of the dermis of cattle skin is characterized by an amide nitrogen content of no more than 1.3 mg per 1 g of dry weight, collagen of at least 90% per dry weight, elastin of no more than 6% per dry weight, sulfated glycosaminoglycans of no more than 0.2 mcg/mg of dry weight, fat of no more than more than 0.5% by dry weight, water not less than 60% by weight and has a thickness of at least 1 mm. A method for obtaining a deamidated collagen biomatrix in the form of a whole fibrous sheet of the dermis of the skin of cattle includes: a) soaking the skins in a solution of soda ash, b) processing the skins with an aqueous solution containing calcium hydroxide and sodium sulfide, c) removing the layer containing the remains of hair follicles, desiccation, cutting into sheets, removing the remains of hair follicles and mezdra, d) processing the sheets with an aqueous solution containing caustic soda and sodium sulfate, e) sequential washing of the obtained deamidated sheets from alkali in an electrolyte solution, in an inorganic acid solution, in water or a buffer solution. The proposed deamidated collagen biomatrix in the form of a single fibrous sheet it has a high degree of deamidation of the side groups of amino acid residues, has a preserved structure of collagen molecules, high technological characteristics, is characterized by the absence of cytotoxicity, local and systemic toxicity, has high biological compatibility, is suitable for creating a wide range of biomedical materials and products, as well as use in cosmetology and the food industry.
EFFECT: has high technological characteristics; has high biological compatibility.
15 cl, 12 dwg, 21 tbl, 7 ex
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