FIELD: biotechnology.
SUBSTANCE: following is proposed: a method of obtaining an antigen from Mycobacterium bovis Bovines-8 strain 700201 with a molecular weight of 24 kDa to study the humoral response, the said method includes cultivating the pathogen on a Levenstein-Jensen solid nutrient medium, destroying the washed cells at a frequency of 22 kHz for 5–10 s, then homogeneous mycobacterial cells are destroyed three times using zirconium beads with a diameter of 0.1 mm in a given time mode, clarified by centrifugation at 10,000 rpm for 10 minutes, the antigen is preparatively fractionated on a 15 cm column in 12% PAGE using sodium dodecyl sulfate at a concentration of 0.1%.
EFFECT: invention provides an addition to the diagnostic panel of biomarkers for studying the humoral immune response and for immunological tests.
1 cl, 6 dwg, 4 ex
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