FIELD: biotechnology.
SUBSTANCE: invention represents a method for obtaining an antigen from Mycobacterium bovis Bovinus-8 strain 700201 with molecular weight of 28 kDa for studying the humoral immune response, involving cultivation of an agent on a solid nutrient medium of Levenshtein-Jensen medium, destruction of washed cells and fractionation by preparative electrophoresis, where washed cells are subjected to destruction three times in homogenizer Fast Prep 24 for 45 sec at vibration rate of 6.5 ms, destructed cells are removed by centrifugation at 7,000 rpm, maintained supernatant at +4–6 °C for 10 days, repeatedly removing spontaneous sediment by centrifugation, adding to supernatant lysing buffer containing 0.2 % sodium dodecyl sulphate, 0.1 % 2-β mercaptoethanol, samples are held at 100 °C from 10 minutes, fractionated on column using 9 % polyacryamide gel.
EFFECT: invention enables to obtain an antigen having high specificity and activity.
1 cl, 5 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF OBTAINING ANTIGEN FROM MYCOBACTERIUM BOVIS BOVINUS-8 STRAIN 700201 WITH MOLECULAR WEIGHT OF 24 KDA FOR STUDYING HUMORAL IMMUNE RESPONSE | 2023 |
|
RU2811034C1 |
| RECOMBINANT PLASMIDE ENCODING HYBRID GST-ESAT-6 POLYPEPTIDE HAVING ACTIVITIES OF SPECIES-SPECIFIC MYCOBACTERIAL ESAT-6 ANTIGEN, RECOMBINANT ESCHERICHIA COLI STRAIN AS PRODUCER OF HYBRID GST-ESAT-6 POLYPEPTIDE AND RECOMBINANT GST-ESAT-6 POLYPEPTIDE | 2004 |
|
RU2282661C2 |
| METHOD FOR PRODUCING ANTIGENE PREPARATION FROM MYCOBACTERIUM TUBERCULOSIS WITH EXTENDED-SPECTRUM SERUM-POSITIVE FRACTIONS IN WESTERN BLOTTING REACTION | 2010 |
|
RU2431675C1 |
| MEDICINE FORMING CELLULAR IMMUNITY FOR MYCOBACTERIUM TUBERCULOSIS H37 Rv, METHOD OF PRODUCTION THEREOF (VERSIONS), RECOMBINANT STRAIN AND TUBERCULOSIS DIAGNOSTIC AGENT | 2007 |
|
RU2341288C1 |
| RECOMBINANT PLASMID DNA pTB323 CODING HYBRID POLYPEPTIDE CST-DELTAMPT64 WITH PROPERTIES OF SPECIES-SPECIFIC MYCOBACTERIAL ANTIGEN MPT64 (MPB64), RECOMBINANT ESCHERICHIA COLI BACTERIAL STRAIN - PRODUCER OF HYBRID POLYPEPTIDE CST-DELTAMPT64 AND RECOMBINANT POLYPEPTIDE CST-DELTAMPT64 | 2011 |
|
RU2458130C1 |
| RECOMBINANT PLASMID DNA pTB232, WHICH CODES HYBRID POLYPEPTIDE GST-CFP10 WITH PROPERTIES OF SPECIES-SPECIFIC MYCOBACTERIAL ANTIGEN CFP10, RECOMBINANT STRAIN OF BACTERIA Escherichia coli-PRODUCER OF HYBRID POLYPEPTIDE GST-CFP10 AND RECOMBINANT POLYPEPTIDE GST-CFP10 | 2008 |
|
RU2381274C2 |
| METHOD OF OBTAINING ANTIGEN FROM MICOBACTERIUM TUBERCULOSIS | 2008 |
|
RU2390559C1 |
| METHOD FOR DETECTING CROSS-REACTING ANTIGENS OF THE AGENTS OF MELIOIDOSIS, GLANDERS, PLAGUE, TULAREMIA AND TUBERCULOSIS | 2004 |
|
RU2286576C2 |
| METHOD FOR DIAGNOSING TUBERCULOSIS AND DIFFERENTIAL DIAGNOSIS OF TUBERCULOSIS AND LATENT TUBERCULOSIS INFECTION | 2014 |
|
RU2576833C1 |
| METHOD OF PRODUCING RABIES VIRUS ANTIGEN FOR SEROLOGICAL DIAGNOSIS | 2018 |
|
RU2694836C1 |
