FIELD: medicine.
SUBSTANCE: recombinant strain 2-9XL Acinetobacter johnsonii as a part of chromosome contains fragments of chromosomal DNA Mycobacterium tuberculosis H37 Rv and ensures simultaneous synthesis of several proteins forming stabile species-specific tuberculosis glycoprotein complex (Tb-antigen) in outer membrane and capsuliferous bacteria substance. Production method of specified Tb-antigen provides use as producer of recombinant strain 2-9XL Acinetobacter johnsonii using standard mediums, cost-effective safe practice for separation and purification of tuberculosis complex o molecular weight within 55.0 to 75.0 kDa and carbohydrate availability within 20 to 50%. Produced Tb-antigen is used to receive agents forming B- and T-cellular immunity for M tuberculosis H37 Rv and tuberculosis diagnostics.
EFFECT: recombinant strain under invention is perspective producer of specified medicine providing synthesis stability of species-specific tuberculosis complex.
5 cl, 14 dwg, 1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| POLYANTIGENIC VACCINE FOR PREVENTING AND ADJUNCTIVE TREATMENT OF TUBERCULOSIS | 2019 |
|
RU2724896C1 |
| HBHA-DETECTION OF TUBERCULOSIS AND MYCOBACTERIUM TUBERCULOSIS INFECTION | 2005 |
|
RU2426128C2 |
| RECOMBINANT PLASMIDE ENCODING HYBRID GST-ESAT-6 POLYPEPTIDE HAVING ACTIVITIES OF SPECIES-SPECIFIC MYCOBACTERIAL ESAT-6 ANTIGEN, RECOMBINANT ESCHERICHIA COLI STRAIN AS PRODUCER OF HYBRID GST-ESAT-6 POLYPEPTIDE AND RECOMBINANT GST-ESAT-6 POLYPEPTIDE | 2004 |
|
RU2282661C2 |
| METHOD FOR DIAGNOSING TUBERCULOSIS AND DIFFERENTIAL DIAGNOSIS OF TUBERCULOSIS AND LATENT TUBERCULOSIS INFECTION | 2014 |
|
RU2576833C1 |
| DIAGNOSTIC TECHNIQUE FOR MYCOBACTERIUM TUBERCULOSIS | 2015 |
|
RU2594063C1 |
| METHOD FOR PRODUCING ANTIGEN FROM MYCOBACTERIUM BOVIS BOVINUS-8 STRAIN 700201 WITH MOLECULAR WEIGHT OF 28 kDa FOR STUDYING HUMORAL IMMUNE RESPONSE | 2018 |
|
RU2691586C1 |
| LIPOSOME FORMULATION SUITABLE FOR TREATING OR PREVENTING TUBERCULOSIS | 2012 |
|
RU2648842C2 |
| COMPOSITIONS AND METHODS FOR TREATING ACTIVE MYCOBACTERIUM TUBERCULOSIS INFECTION | 2013 |
|
RU2659149C2 |
| EXPRESS METHOD FOR DIAGNOSING TUBERCULOSIS USING LATEX AGGLUTINATION REACTION | 1998 |
|
RU2130615C1 |
| RECOMBINANT PLASMID DNA pTB232, WHICH CODES HYBRID POLYPEPTIDE GST-CFP10 WITH PROPERTIES OF SPECIES-SPECIFIC MYCOBACTERIAL ANTIGEN CFP10, RECOMBINANT STRAIN OF BACTERIA Escherichia coli-PRODUCER OF HYBRID POLYPEPTIDE GST-CFP10 AND RECOMBINANT POLYPEPTIDE GST-CFP10 | 2008 |
|
RU2381274C2 |
