METHOD FOR PRODUCING ANTIGENE PREPARATION FROM MYCOBACTERIUM TUBERCULOSIS WITH EXTENDED-SPECTRUM SERUM-POSITIVE FRACTIONS IN WESTERN BLOTTING REACTION Russian patent published in 2011 - IPC C12P21/00 A61K39/04 

FIELD: medicine.

SUBSTANCE: method for producing an antigene preparation from Micobacterium tuberculosis involves mycobacteria cultivation on a Lowenstein-Jensen medium, three-fold washing of bacterial cells from the residual nutrient medium. The washed bacterial mass is treated by aqueous acetone solutions of the concentrations 25 % and 50 %, and each acetone treatment the cells are precipitated by centrifugation. Further, the extracts containing an antigen are produced by three-fold sequential treatment of the bacterial mass deposited by centrifugation, by aqueous dimethylsulphoxide solutions with the increasing concentrations 5 % to 20 % with an insoluble fraction every time separated by centrifugation. The produced three portions of dimethylsulphoxide extracts are mixed and exposed to in-depth dialysis against distilled water. The prepared solution is used as an antigen-containing material for preparing a nitrocellulose membrane immunosorbent.

EFFECT: antigen preparation according to the invention exhibits extended-spectrum serum-positive fractions in a western blotting reaction.

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