NEW POLYPEPTIDE AND METHOD OF PRODUCING L-LEUCINE USING ITS Russian patent published in 2024 - IPC C12N9/10 C12N15/54 C12N1/21 C12N15/77 C12P13/06 

FIELD: biotechnology.

SUBSTANCE: following is proposed: a mutated polypeptide with isopropyl malate synthase activity containing the amino acid sequence SEQ ID NO: 1 or SEQ ID NO: 16, where the proline at position 247 or 212, respectively, is replaced by cysteine. Also the following is proposed: a mutated polypeptide having isopropyl malate synthase activity containing the amino acid sequence SEQ ID NO: 1, where (1) proline at position 247 is replaced by cysteine and (2) where arginine at position 558 is replaced by histidine and/or where glycine at position 561 is replaced by aspartic acid. Besides, the following is proposed: a polynucleotide encoding the said mutated polypeptide, an expression vector containing the said polynucleotide, a microorganism capable of producing L-leucine, a composition for producing L-leucine and a method of producing L-leucine.

EFFECT: invention makes it possible to obtain L-leucine with high yield.

13 cl, 10 tbl, 4 ex