METHODS OF PRODUCING AND USING GUIDE AND NAVIGATION CONTROL PROTEINS Russian patent published in 2024 - IPC C07K16/18 A61K39/395 C07K16/28 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and genetic engineering and represents a method for preparing a therapeutic composition for treating a tumour, comprising cells expressing ROR1, CD19 or a combination thereof, involving: providing a cell material containing a cytotoxic cell, incubating the cell material with a first GNC protein to obtain an activated cell composition, wherein the activated cell composition comprises a first therapeutic cell, wherein the first GNC protein comprises a first cytotoxic binding moiety and a first tumour targeting moiety, wherein the first cytotoxic binding fragment has specificity for the first cytotoxic cell receptor and is configured to activate the first cytotoxic cell by binding to the first cytotoxic cell receptor, and wherein the first tumour targeting fragment has specificity for the first tumour cell receptor, and wherein the first therapeutic cell comprises a first GNC protein bound to the cytotoxic cell through a binding interaction with the first cytotoxic cell receptor, and formulating an activated cell composition to obtain a therapeutic composition, wherein the therapeutic composition substantially does not contain exogenous viral and non-viral DNA or RNA.

EFFECT: invention enables to treat a subject with a tumour with a higher degree of effectiveness.

38 cl, 27 dwg, 4 tbl, 5 ex