EXPRESSION VECTOR BASED ON HUMAN ADENOVIRUS 19 SEROTYPE AND METHOD OF ITS APPLICATION Russian patent published in 2024 - IPC C12N15/861 A61K45/00 

FIELD: biotechnology; immunology; virology.

SUBSTANCE: group of inventions relates to biotechnology, immunology and virology. An expression vector based on the human adenovirus genome of serotype 19 has been created, containing: a constant sequence with deletion of E1 and E3 regions, inside which there is an expression cassette with the target gene; a variable region at the right end of the genome up to 1000 bp in size. In a particular embodiment of the expression vector, the constant sequence to the expression cassette has the sequence SEQ ID NO:1, and after the expression cassette has the sequence SEQ ID NO:2. The expression cassette as a vaccine antigen gene may contain SEQ ID NO:3, or SEQ ID NO:4, or SEQ ID NO:5, or SEQ ID NO:6, or SEQ ID NO:7. A method for obtaining an expression vector is also described, characterized in that it uses a shuttle plasmid containing a sequence that is homologous to a section of the adenovirus genome, the serotype of which differs from 19.

EFFECT: group of inventions provides the creation of an expression vector based on human adenovirus 19 serotype, which is capable of inducing an immune response to the vaccine antigen.

8 cl, 10 dwg, 5 tbl, 9 ex