FIELD: biotechnology.
SUBSTANCE: following is described: a method of indirectly determining the titer of infectious activity of the causative agent of canine parvovirus enteritis in raw materials for vaccines using real-time PCR, characterized by its involving the use of developed original oligonucleotide primers and a probe.
EFFECT: development of a highly sensitive, highly specific, fast and objective method, which does not involve the use of cell lines, for indirectly determining the titer of infectious activity of the causative agent of canine parvovirus enteritis in raw materials for vaccines using real-time PCR.
2 cl, 2 dwg, 6 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETERMINING CONCENTRATION OF VIRIONS OF CAUSATIVE AGENT OF CANINE PARVOVIRAL ENTERITIS IN RAW MATERIAL FOR VACCINES | 2023 |
|
RU2827231C1 |
| METHOD FOR INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CANINE MASTADENOVIRUS IN RAW MATERIALS FOR VACCINES USING REAL-TIME PCR | 2023 |
|
RU2808585C1 |
| METHOD OF INDIRECTLY DETERMINING TITER OF INFECTIOUS ACTIVITY OF CANINE INFECTIOUS HEPATITIS VIRUS GENOTYPE CAV-1 IN RAW MATERIALS FOR CULTURED VACCINES USING REAL-TIME POLYMERASE CHAIN REACTION USING QUANTITATIVE ASSESSMENT CYCLE Cq | 2023 |
|
RU2812441C1 |
| METHOD OF INDIRECT DETERMINING OF TITRE OF INFECTIOUS ACTIVITY OF CAUSATIVE AGENT OF CANINE DISTEMPER IN RAW MATERIALS FOR VACCINES USING METHOD OF REVERSE TRANSCRIPTION AND AMPLIFICATION REACTION IN REAL TIME | 2023 |
|
RU2815533C1 |
| METHOD FOR GENOTYPING ISOLATES AND STRAINS OF CAUSATIVE AGENT OF CANINE PARVOVIRAL ENTERITIS BASED ON PHYLOGENETIC ANALYSIS USING ORIGINAL OLIGONUCLEOTIDE PRIMERS FOR HIGHLY VARIABLE REGION OF VP GENE | 2023 |
|
RU2822036C1 |
| METHOD FOR INDIRECT DETERMINATION OF FELINE CALICIVIRUS INFECTIOUS ACTIVITY TITRE IN NON-INACTIVATED RAW MATERIAL FOR CULTURAL VACCINES BY MEANS OF QUANTITATIVE REGISTRATION OF REACTION OF AMPLIFICATION OF ORF1-GENE SECTION OF VIRAL RNA | 2023 |
|
RU2821027C1 |
| ASSOCIATED VACCINE AGAINST CANINE DISTEMPER, PARVOVIRUS AND CORONAVIRUS ENTERITIS, ADENOVIRUS INFECTION OF DOGS | 2022 |
|
RU2806164C1 |
| ASSOCIATED VACCINE AGAINST DISTEMPER, PARVOVIRUS AND CORONAVIRUS ENTERITIS, ADENOVIRUS INFECTION AND CANINE RABIES | 2023 |
|
RU2817255C1 |
| METHOD FOR INDIRECT DETERMINATION OF CONCENTRATION OF A-ANTIGEN OF INDUSTRIAL STRAIN "VGNKI" OF CAUSATIVE AGENT OF INFECTIOUS HEPATITIS OF DOGS IN RAW MATERIAL FOR CULTURAL VACCINES ACCORDING TO QUANTITATIVE ASSESSMENT CYCLE DURING AMPLIFICATION OF TARGET REGION OF ORF25-GENE | 2023 |
|
RU2823930C1 |
| METHOD OF INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CAUSATIVE AGENT OF INFECTIOUS FELINE RHINOTRACHEITIS FHV IN NON-INACTIVATED RAW MATERIALS FOR VACCINES USING POLYMERASE CHAIN REACTION AND QUANTITATIVE HYBRIDIZATION-FLUORESCENCE DETECTION IN REAL TIME | 2023 |
|
RU2808641C1 |
