FIELD: veterinary science; virology.
SUBSTANCE: described is a method for molecular diagnostics, namely for genotyping isolates and strains of the causative agent of canine parvoviral enteritis based on phylogenetic analysis using original oligonucleotide primers for a highly variable region of the VP2 gene. Developed method was used for the first time to solve this problem. It was found that in the analysed DNA region of the CPV in range of 4000…5307 bp there is a maximum number of nucleotide substitutions, average value of substitutions is 28.56±1.21%, which is 25.53-28.03% higher in comparison with the prototype method amplicons. This made it possible to develop original high-specific oligonucleotide primers 556 for and 555 rev_mod for detection of this genome region with size of 1308 bp by means of amplification reaction. For the first time, identification of nucleotide sequences of this genome region of field isolates and vaccine strains of the causative agent of canine parvoviral enteritis for phylogenetic analysis is presented.
EFFECT: developed method is specific with respect to the CPV genome, which is confirmed by research.
3 cl, 2 dwg, 5 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CANINE MASTADENOVIRUS IN RAW MATERIALS FOR VACCINES USING REAL-TIME PCR | 2023 |
|
RU2812858C1 |
| METHOD FOR GENOTYPING FELINE CALICIVIRUS ISOLATES AND STRAINS BY PHYLOGENETIC ANALYSIS USING ORIGINAL OLIGONUCLEOTIDE PRIMERS FOR HIGHLY VARIABLE REGION OF ORF2-ORF3 GENES | 2023 |
|
RU2822162C1 |
| METHOD FOR DIFFERENTIATION OF VACCINE STRAIN "VGNKI" OF ADENOVIRAL INFECTION CAUSATIVE AGENT FROM OTHER STRAINS AND FIELD ISOLATES BY ANALYZING MAXIMUM MELTING POINTS OF PCR PRODUCTS USING SUPERNOVA v605 TANDEM FLUORESCENT DYE | 2023 |
|
RU2825893C1 |
| METHOD OF DIFFERENTIATING VACCINE STRAIN "RICH" FROM OTHER STRAINS AND FIELD ISOLATES OF CAUSATIVE AGENT OF CORONAVIRUS INFECTION IN DOGS BY ANALYZING MAXIMUM EXTREMA OF AMPLICON MELTING TEMPERATURE CURVES USING SUPERNOVA v428 VIOLET LASER DYE | 2023 |
|
RU2826458C1 |
| ARCHIE STRAIN OF VIRUS CARNIVORE PROTOPARVOVIRUS 1 OF PARVOVIRAL ENTERITIS OF DOGS FOR PRODUCING BIOPREPARATIONS FOR DIAGNOSING AND SPECIFIC PREVENTION OF CANINE PARVOVIRUS ENTERITIS | 2023 |
|
RU2817267C1 |
| ASSOCIATED VACCINE AGAINST DISTEMPER, PARVOVIRUS AND CORONAVIRUS ENTERITIS, ADENOVIRUS INFECTION AND CANINE RABIES | 2023 |
|
RU2817255C1 |
| METHOD FOR DIFFERENTIATION OF LAVR VACCINE STRAIN OF INFECTIOUS RHINOTRACHEITIS AGENT FROM OTHER STRAINS AND FIELD ISOLATES OF FELINE INFECTIOUS RHINOTRACHEITIS VIRUS BY ANALYSING MELTING TEMPERATURE CURVE OF PCR V1-GENE PRODUCTS USING ORANGE POLYMER DYE SUPERNOVA V605 | 2024 |
|
RU2824661C1 |
| ASSOCIATED VACCINE AGAINST CANINE DISTEMPER, PARVOVIRUS AND CORONAVIRUS ENTERITIS, ADENOVIRUS INFECTION OF DOGS | 2022 |
|
RU2806164C1 |
| METHOD FOR DIAGNOSIS OF CATTLE LEUKEMIA VIRUS | 2018 |
|
RU2694966C1 |
| METHOD FOR INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CANINE MASTADENOVIRUS IN RAW MATERIALS FOR VACCINES USING REAL-TIME PCR | 2023 |
|
RU2808585C1 |
