FIELD: veterinary science; virology.
SUBSTANCE: described is a method for molecular diagnostics, namely for genotyping isolates and strains of the causative agent of canine parvoviral enteritis based on phylogenetic analysis using original oligonucleotide primers for a highly variable region of the VP2 gene. Developed method was used for the first time to solve this problem. It was found that in the analysed DNA region of the CPV in range of 4000…5307 bp there is a maximum number of nucleotide substitutions, average value of substitutions is 28.56±1.21%, which is 25.53-28.03% higher in comparison with the prototype method amplicons. This made it possible to develop original high-specific oligonucleotide primers 556 for and 555 rev_mod for detection of this genome region with size of 1308 bp by means of amplification reaction. For the first time, identification of nucleotide sequences of this genome region of field isolates and vaccine strains of the causative agent of canine parvoviral enteritis for phylogenetic analysis is presented.
EFFECT: developed method is specific with respect to the CPV genome, which is confirmed by research.
3 cl, 2 dwg, 5 tbl, 4 ex
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Authors
Dates
2024-06-28—Published
2023-08-28—Filed