FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and production of culture vaccines. Described is a method for indirect determination of the infectious activity titre of feline calicivirus in non-inactivated raw material for culture vaccines by means of quantitative registration of reaction of amplification of ORF1-gene section of viral RNA. Main advantages of the present invention are the ability to reduce the time for analysing virus-containing suspensions for determining the titre of the infectious activity of feline calicivirus to 2.5 hours; eliminate the probability of contamination; increase objectivity of analysis; increase the specificity of the analysis due to the use of highly specific original RNA primers and a molecular beacon probe; increase analysis sensitivity due to amplification of only viral RNA molecules; reduce the cost of the analysis method due to the absence of using cell cultures as test systems; increase the reliability of the analysis due to the establishment of the relationship between the titre of the infectious activity of the feline calicivirus and the cycles of the quantitative assessment of the RNA amplification reaction.
EFFECT: analytical sensitivity of the developed method is 0,2 lg TCD50/cm3 with reliability of determining the analyte equal to 95,56%; in 95% confidence interval diagnostic sensitivity was 97,96–99,93%, diagnostic specificity – 98,26–100%, overall accuracy – 98,72–99,96%.
2 cl, 1 dwg, 4 tbl, 6 ex
