FIELD: biotechnology.
SUBSTANCE: method for indirectly determining the titre of infectious activity of the causative agent of canine distemper in raw materials for vaccines by the method of reverse transcription and amplification reaction in real time using a developed logarithmic function of the relationship between the magnitude of the quantitative assessment cycle and the titre of infectious activity of the causative agent of canine distemper. The main advantages of the proposed invention are the ability to eliminate the possibility of contamination during the reaction and subjectivity when taking into account the results of the analysis; reduce the time for analysing virus-containing raw materials for vaccines to 3.5 hours; to increase the specificity and sensitivity of the analysis for determining the titre of infectious activity of the causative agent of canine distemper. In the invention proposed, a relationship is established between the titre of infectious activity of the canine distemper virus and the quantitative assessment cycle, reflected in the form of a logarithmic function, which makes it possible to determine the titre of infectious activity of CDV in raw materials for vaccine production.
EFFECT: invention allows to quickly and with a high degree of reliability calculate the titre of infectious activity of CDV in raw materials for vaccines based on the developed method using original specific oligonucleotide primers and a probe for the CDV H-gene region and a developed logarithmic model.
2 cl, 2 dwg, 6 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETERMINING TITRE OF INFECTIOUS ACTIVITY OF CANINE DISTEMPER AGENT IN RAW MATERIAL FOR MAKING CULTURE INACTIVATED VACCINES | 2024 |
|
RU2831062C1 |
| METHOD FOR MEDIATED DETERMINATION OF RIBONUCLEOPROTEIN CONCENTRATION OF CANINE DISTEMPER AGENT IN RAW MATERIAL FOR VACCINES BY DATA OF QUANTITATIVE ASSESSMENT CYCLE DURING N-GENE AMPLIFICATION | 2023 |
|
RU2829837C1 |
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|
RU2830983C1 |
| METHOD FOR INDIRECT DETERMINING OF THE TITER OF INFECTIOUS ACTIVITY OF FELINE CALICIVIRUS IN RAW MATERIALS FOR VACCINE USING REAL-TIME PCR | 2023 |
|
RU2809221C1 |
| METHOD FOR INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CANINE MASTADENOVIRUS IN RAW MATERIALS FOR VACCINES USING REAL-TIME PCR | 2023 |
|
RU2808585C1 |
| METHOD OF INDIRECTLY DETERMINING TITER OF INFECTIOUS ACTIVITY OF CANINE INFECTIOUS HEPATITIS VIRUS GENOTYPE CAV-1 IN RAW MATERIALS FOR CULTURED VACCINES USING REAL-TIME POLYMERASE CHAIN REACTION USING QUANTITATIVE ASSESSMENT CYCLE Cq | 2023 |
|
RU2812441C1 |
| METHOD FOR INDIRECT DETERMINATION OF FELINE CALICIVIRUS INFECTIOUS ACTIVITY TITRE IN NON-INACTIVATED RAW MATERIAL FOR CULTURAL VACCINES BY MEANS OF QUANTITATIVE REGISTRATION OF REACTION OF AMPLIFICATION OF ORF1-GENE SECTION OF VIRAL RNA | 2023 |
|
RU2821027C1 |
| METHOD OF INDIRECT DETERMINATION OF TITER OF INFECTIOUS ACTIVITY OF CAUSATIVE AGENT OF INFECTIOUS FELINE RHINOTRACHEITIS FHV IN NON-INACTIVATED RAW MATERIALS FOR VACCINES USING POLYMERASE CHAIN REACTION AND QUANTITATIVE HYBRIDIZATION-FLUORESCENCE DETECTION IN REAL TIME | 2023 |
|
RU2808641C1 |
| METHOD OF INDIRECT DETERMINING OF TITER OF INFECTIOUS ACTIVITY OF CANINE ALPHA-CORONAVIRUS PRODUCTION STRAIN NAMED RICH IN RAW MATERIALS FOR VACCINE USING REAL-TIME RT-PCR | 2022 |
|
RU2809224C1 |
| METHOD FOR INDIRECT DETERMINATION OF INFECTIOUS ACTIVITY TITRE OF CAUSATIVE AGENT OF INFECTIOUS FELINE RHINOTRACHEITIS IN RAW MATERIAL FOR VACCINE USING SECOND-ORDER DIFFERENTIAL OF POINT OF INTERSECTION OF LOGISTIC CURVE OF AMPLIFICATION REACTION OF UL35 GENE REGION | 2023 |
|
RU2823781C1 |
