FIELD: biotechnology.
SUBSTANCE: invention relates to a method of obtaining a population of immunoreactive T-cells containing a T-cell receptor (TCR) specific for the peptide:MHC complex, where the peptide of the peptide:MHC complex is a mutant GATA3 peptide sequence. The method involves bringing a population of immune cells into contact with a polynucleotide containing a sequence encoding a polypeptide containing the said mutant GATA3 peptide sequence, or a polypeptide containing the said mutant GATA3 peptide sequence. The immune cell population is a population of immune cells lacking cells expressing CD14 and CD25, and wherein the immune cell population includes a population of FMS-like receptor tyrosine kinase ligand 3 (FLT3L) stimulated antigen presenting cells (APCs) and a population of T-cells.
EFFECT: invention is effective for obtaining a population of immunoreactive T-cells for cancer immunotherapy.
9 cl, 54 dwg, 44 tbl, 41 ex
| Title | Year | Author | Number |
|---|---|---|---|
| ANTIBODIES TO PD-1 AND THEIR APPLICATION | 2017 |
|
RU2761640C2 |
| COMPOSITIONS AND METHODS FOR TREATING AND PREVENTING INFECTIONS CAUSED BY STAPHYLOCOCCUS AUREUS | 2015 |
|
RU2764981C1 |
| USE OF ANTIBODIES TO SCLEROSTIN FOR TREATING OSTEOGENESIS IMPERFECTA | 2017 |
|
RU2789033C2 |
| HPV-SPECIFIC BINDING MOLECULES | 2018 |
|
RU2804664C2 |
| PROCOAGULANT ANTIBODIES | 2018 |
|
RU2810094C2 |
| ARTIFICIAL ANTIGEN-PRESENTING CELLS AND METHODS FOR THEIR APPLICATION | 2018 |
|
RU2763798C1 |
| MODIFIED IGG ANTIBODIES WHICH BIND TO A TRANSFORMING GROWTH FACTOR BETA-1 WITH HIGH AFFINITY, AVIDITY AND SPECIFICITY | 2016 |
|
RU2728858C2 |
| COMPOSITIONS AND METHODS FOR IMMUNE ONCOLOGY | 2016 |
|
RU2771624C2 |
| T-CELL RECEPTORS | 2018 |
|
RU2762255C2 |
| IMMUNITY INDUCER | 2016 |
|
RU2758112C2 |
