FIELD: biotechnology.
SUBSTANCE: invention relates to a method of ex vivo expansion of natural killer (NK) cells. The method includes (a) obtaining a source population of mononuclear cells (MNC) from umbilical cord blood; (b) stimulation of MNC in the presence of antigen presenting cells (APC) and IL-2; and (c) restimulation of cells using APC to produce expanded NK cells. The method is performed in a bioreactor. The APCs used are engineered to express membrane-bound IL-21 (mbIL-21) and are universal antigen presenting cells (uAPCs) engineered to express (1) CD48 and/or CS1 (CD319), (2) membrane-bound interleukin-21 (mbIL-21) and (3) 41BB ligand (41BBL).
EFFECT: invention provides rapid expansion of natural killer cells from umbilical cord blood in clinically significant doses and without the need for HLA compatibility between donor and recipient for the treatment of myeloma and lymphoma.
54 cl, 4 dwg, 4 tbl, 1 ex
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