OLIGONUCLEOTIDES FOR DIAGNOSING EPILEPSY BY QUANTITATIVE PCR Russian patent published in 2024 - IPC C12Q1/68 C12Q1/686 

FIELD: biotechnology.

SUBSTANCE: disclosed is a set of oligonucleotides for determining microRNA hsa-miR-134-5p, hsa-miR-106b-5p, hsa-miR-191 and analysis of relative expression of microRNA data in blood plasma. Set has the following composition: oligonucleotides for analysing microRNA-134-5p expression, oligonucleotides for analysing microRNA-106b-5p expression and oligonucleotides for analysing microRNA-191 expression. Following is used to analyse microRNA-134-5p expression: reverse transcription primer CAGGCGATAGTCCGATGCATGCGCATGCATCGGACTATCGCCTGTTGGTGACTAGGT, forward F-primer TCCTGTGGGCCACCTAGTCAC, R-primer CGTACGTAGCCTGATAGCGGAC, probe FAM CAACAGGCGATAGTCCGATGCATGC BHQ1. For analysis of microRNA-106b-5p expression using: primer for reverse transcription CAGGCGATAGTCCGATGCATGCGCATGCATCGGACTATCGCCTGATCTGCACTGTCA, F-primer TGACAGTCGTGAAATAATGTATGTC, R-primer CGTACGTAGCCTGATAGCGGAC, probe FAM CAGAT GTCGTATCCAGTGCGAATAC BHQ1. For analysis of microRNA-191 expression using: primer for reverse transcription CAGGCGATAGTCCGATGCATGCGCATGCATCGGACTATCGCCTGGGGGACGAAATC, F-primer GCTTTAGGTTCGCGTCCTGT, R-primer CGTACGTAGCCTGATAGCGGAC, probe FAM CCCCGTCGTATCCAGTGCGAATAC BHQ1. MicroRNA-191 is a reference gene, relative to which expression of microRNA 134-5p, microRNA 106b-5p is analysed.

EFFECT: high specificity of quantitative analysis of microRNA, reduced time spent on analysis; at the same time diagnostics is differentiated and allows to determine not only presence of epilepsy, but also its type: thus, microRNA hsa-miR-106b-5p is used as a biomarker of juvenile myoclonic epilepsy, and microRNA hsa-miR-134-5p – as a biomarker of mesial temporal lobe epilepsy.

1 cl, 2 dwg, 2 ex