METHOD FOR DIAGNOSING GLIAL BRAIN TUMORS OF HIGH DEGREE OF MALIGNANCY Russian patent published in 2021 - IPC G01N33/48 C12Q1/68 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to molecular oncology, and can be used for high-grade gliomas diagnostics. That is ensured by analyzing the expression of micro-RNA in a plasma sample involving exogenous control in a plasma sample and recovery of total RNA, reverse transcription followed by amplification in real time. High-specific primers for hsa-miR-22-3p, hsa-miR-122-5p, hsa-miR-107, hsa-miR-324-5p, hsa-miR-155-5p, hsa-miR-21-5p, hsa-miR-330-3p, hsa-miR-146a-5p, cel-miR-39-5p.The obtained data are analyzed and coefficients of relative expression are calculated RE₂₂: hsa-miR-22-3p, RE₁₂₂: hsa-miR-122-5p, RE₁₀₇: hsa-miR-107, RE₃₂₄: hsa-miR-324-5p, RE₁₅₅: hsa-miR-155-5p, RE₂₁: hsa-miR-21-5p, RE₃₃₀: hsa-miR-330-3p, RE_146a: hsa-miR-146a-5p using reference control cel-miR-39 by formula: RE_(miR)=log₂ 2^{-(CtmiR-Ct39)}, where Ct is the cycle number obtained as a result of semi-quantitative RT-PCR, miR- is one of the analyzed micro-RNAs, 39 is cel-miR-39-5p. With values RE₂₂> - 3, RE₁₂₂> - 3.35, RE₁₀₇> - 4.5, RE₃₂₄> - 7.5, RE₁₅₅> - 9.5, RE₂₁> - 1.5, RE₃₃₀> - 8.5, RE_146a> - 8.5, high-grade glial tumor is diagnosed.

EFFECT: method enables diagnosing glioma of high degree of malignancy without surgical intervention in a short period of time.

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