FIELD: chemistry.
SUBSTANCE: invention relates to a method for synthesis of 4'-ethynyl-2'-deoxynucleoside, comprising combining compound 6.5
with purine nucleoside phosphorylase and a nucleobase in a buffer solution containing a manganese (II) salt, where 2X+ is (a) two protons, (b) one proton and one monovalent cation, (c) two monovalent cations, where each cation is the same or they are different from each other, or (d) one divalent cation, and where 4'-ethynyl-2'-deoxynucleoside is
.
Invention also relates to novel intermediate compounds and methods for production thereof.
EFFECT: novel method of producing 4'-ethynyl-2'-deoxynucleoside, which eliminates the use of protective groups on intermediate compounds, improves stereoselectivity of glycosylation and reduces the number of process steps.
32 cl, 3 tbl
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