FIELD: process engineering.
SUBSTANCE: invention relates to biotechnology and discloses the recombinant expression vector. It comprises: either sequence encoding the phosphorylase of purine nucleosides (PNPhase of Sulfolobus solfataricus), or sequence encoding uridine phosphorylase (UPhase of Aeropyrum pernix), or both said sequences. Each said sequences is functionally related with one or more regulatory sequence that controls the creation of said phosphorylase in hostcell (E.coil). Invention discloses also hostcell including said expression vector and lysate resulted from hostcell lysing. This invention discloses also the transglycosylation between nucleoside which makes the sugar donor and acceptor base in the presence of phosphate-ions and any of said ferments or combinations thereof.
EFFECT: new recombinant nucleosides of higher thermal stability and catalytic activity for transglycosylation reactions.
13 cl, 8 dwg, 4 tbl, 13 ex
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