FIELD: organic chemistry, biotechnology, pharmacy. SUBSTANCE: invention relates to manufacturing antiviral compounds. Method for preparing ribavirin involved enzymatic trans-glycosylation reaction of 1,2,4-triazole-3-carboxamide followed by isolation of the end product. The trans-glycosylation reaction is carried out in the presence of recombinant purine-nucleoside phosphorylase from E. coli BL21 (DE3)/pERPUPHO1 immobilized on the aminopropylated silochrome. Method provides simplifying technology and using enzyme for 15 industrial cycles. EFFECT: improved preparing method. 4 dwg, 2 ex
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