MULTIMERIZING POLYPEPTIDES DERIVED FROM DOMAIN WITH ROLL-TYPE PENTONE BASE OF ADENOVIRUS Russian patent published in 2024 - IPC C12N7/00 C07K14/05 A61K39/12 A61P31/12 A61P35/00 A61P37/00 

FIELD: biotechnology.

SUBSTANCE: described is a polypeptide for presentation of oligopeptide, polypeptide, protein and / or protein complex, having structure A-L₁-B-L₂-C (formula (I)), where A is the N-terminal amino acid portion of the adenovirus pentone base protein, B is the adenovirus pentone base amino acid portion, C is the adenovirus pentone base C-terminal amino acid portion, where B is an amino acid region located between A and C in the sequence of said adenovirus pentone base, where A, B and C form a roll-type domain (jellyrollfold) of said pentonaadenovirus base protein, L₁ and L₂ are independently selected from a group consisting of an oligopeptide, a polypeptide, a protein and a protein complex, where said oligopeptide, polypeptide, protein and protein complex, respectively, are either substantially non-adenoviral, or if adenoviral, A-L₁-B-L₂-C forms a pentone base chimera, where A, B and C form a roll-type domain of the adenovirus pentone base of one adenovirus subtype, and L₁ and L₂ form a "crown" domain of a pentone base of adenovirus subtype different from the subtype adenovirus roll-type domain. Described is a nucleic acid coding said polypeptide. Disclosed is nucleic acid for producing nucleic acid by inserting nucleotide sequences coding L₁ and L₂ containing a sequence having following common 5'-a-is₁-l₁-is₂-b-is₃-l₂-is₄-c-3', where a is a nucleotide sequence coding A of formula (I), b is a nucleotide sequence coding B of general formula (I), c is a nucleotide sequence coding C of general formula (I), and l₁, l₂ each represents a nucleotide sequence, is 1 to is 4 each independently represents a nucleotide sequence containing at least one insertion site. Disclosed vector to obtain said polypeptide. Described is a recombinant host cell for producing said polypeptide. Described is a pentameric polypeptide complex for presenting an oligopeptide, a polypeptide, a protein and/or a protein complex, where A-L₁-B-L₂-C forms a pentone base chimera and where A, B and C form roll-type domainadenovirus pentone base of one adenovirus subtype, and L₁ and L₂ form a "crown" domain of adenovirus subtype of adenovirus subtype different from the adenovirus subtype of the roll-type domain. Disclosed is a virus-like particle (VLP) for the presentation of an oligopeptide, a protein and / or a protein complex containing 12 pentameric complexes. Disclosed is a pharmaceutical composition for the presentation of an oligopeptide, a protein and/or a protein complex containing a polypeptide, a nucleic acid, a vector, a host cell or HPV together with at least one pharmaceutically acceptable carrier, excipient and/or diluent. Disclosed is a method of producing a polypeptide. Polypeptide frameworks according to the invention are intended for optimal presentation of peptides, polypeptide sequences, protein domains, proteins and protein complexes consisting of two, several or many subunits.

EFFECT: such oligopeptides, polypeptide sequences, protein domains and proteins represented by polypeptide scaffolds according to the present invention may include antigenic particles which stimulate the immune system to elicit an immune response, for example, for vaccination purposes, or for producing antibodies or other binding molecules in a cell culture, or in a test tube.

36 cl, 5 dwg, 5 tbl, 1 ex