METHOD FOR DIAGNOSING DESULFOVIBRIO SPECIES IN GASTROINTESTINAL MICROBIOCENOSIS DISORDERS Russian patent published in 2024 - IPC C12Q1/04 C12N1/20 

FIELD: microbiology.

SUBSTANCE: disclosed is a method for diagnosing Desulfovibrio spp. in case of gastrointestinal microbiocenosis disorders. Method comprises sampling biological material, preparing a nutrient medium for culturing bacteria of the genus Desulfovibrio spp., preparation of a suspension of biological material 1 g, preparation of tenfold dilutions of the suspension of biological material, sowing 100 mcl of dilutions of the suspension into a nutrient medium for cultivating bacteria of the genus Desulfovibrio spp., incubation of the obtained mixture in anaerobic conditions for 120–170 hours at 37 °C. Visual assessment of growth of bacteria of the genus Desulfovibrio spp. produced by the presence or absence of blackening of the nutrient medium, where the presence of blackening indicates the presence of bacteria of the genus Desulfovibrio spp. in a sample of biological material, and in the absence of blackening of the nutrient medium, the result is recorded as not containing bacteria of the genus Desulfovibrio spp.

EFFECT: invention provides higher quality of diagnostics in patients with disturbed microbiocenosis of gastrointestinal tract with reduction of time and labour costs, including in conditions of polymicrobial community.

4 cl, 1 tbl, 6 ex