METHOD FOR DETECTION OF SURROGATE MARKERS OF MUTATIONS OF GENES OF VARIABLE REGIONS OF HEAVY CHAINS OF IMMUNOGLOBULINS IN CHRONIC LYMPHOCYTIC LEUKEMIA Russian patent published in 2024 - IPC C12Q1/68 C12N15/13 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to oncohaematology, and concerns detection of molecular-genetic criteria of unfavourable prognosis of chronic lymphocytic leukemia in onset of disease. What is presented is a method for detecting surrogate markers of mutations in variable regions of immunoglobulin heavy chains. Method involves extraction of genomic DNA from leukocytes of peripheral venous blood of patients, genotyping of polymorphism of loci IL4-590C>T and TLR9-1237T>C, evaluation of the distribution of the identified alleles and genotypes. In case of detection of wild type allele and genotypes containing it, IL4 locus, mutant allele and genotypes containing it, TLR9 gene, high risk of progressive clinical course of CLL accompanied by unfavourable cytogenetic abnormalities, resistant/recurrent, aggressive clinical course, short event-free and overall survival with no clonal IGHV-gene mutations is predicted.

EFFECT: invention enables to predict the clinical course of CLL at an early stage of the disease, is available for wide application, determines the choice of risk-adapted treatment methods.

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