CONSTRUCTED PAIRS OF HEAVY-LIGHT CHAINS OF IMMUNOGLOBULIN AND USE THEREOF Russian patent published in 2024 - IPC C07K16/46 C12N15/13 C12N15/63 C12N5/10 C12P21/08 

FIELD: biotechnology.

SUBSTANCE: invention relates to nucleic acids coding antigen-binding polypeptide constructs and can be used for recombinant production of antigen-binding polypeptide construct in mammalian cells. Disclosed are sets of polynucleotides for producing an isolated antigen-binding polypeptide structure, wherein the set of polynucleotides codes an antigen-binding polypeptide structure containing at least a first heterodimer which binds a first epitope, and a second heterodimer which binds a second epitope.

EFFECT: invention provides preferential pairing of heavy chains with specific light chains in heterodimeric structures, which provides expression of a bispecific monoclonal antibody (Mab) with negligible or limited mispairing, and minimizes the need to purify desired heterodimers from undesirable or mistakenly coupled products, heterodimers can also exhibit thermal stability comparable to that of heterodimers which do not contain amino acid modifications, and exhibit antigen binding affinity comparable to heterodimers which do not contain amino acid modifications.

22 cl, 19 dwg, 31 tbl, 15 ex